Naslov
A computorized method of somatosenosory evoked potentials monitoring: II. Advantage of the model for neuropathy studies in experimental diabetes

Autori
Bjegović, Milica ; Išgum, Velimir ; Slijepčević, Milivoj

Izvornik
Periodicum biologorum (0031-5362) 98 (1996), 1; 49-54

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Alloxan diabetes ; Neuropathy studies ; Noninvasive method ; Rodents ; Somatosensory evoked potentials

Sažetak
Background and purpose: SEPs in rats and mice are, as we previously demonstrated, to be correspodent and comparable regardless the species, strain, sex and age. Therefore, it was assumed that general uniformity of the main SEPs components in healthy and diabetic rodents opened the new possibilities of investigations as a privilege model for neuropathologic studies. Thanking to our model, the neuropathological consequences of development of chemically induced experimental diabetes could be continously followed by repeated SEPs measurement in the same animal before and after evocation of the disease and therapy, what privileged our model for the late complication studies in diabetes research. Material and methods: Wistar female and male rats of different age and various duration of diabetes and CBA/H Zgr mice were used. SEPs were monitored and followed in time by the means of the special computorized program for recording and analysis of SEPs in a manner reported previously (6). Results: Two months after alloxan treatment permanent hyperglycaemia in diabetic CBA/H Zgr mice resulated in marked amplitude increase and slight latency prolongation of P22 component in the SEPs brain wave complex, accompanied by the 75% reduction of N27 amplitude peak. Slight latency increase was seen in N11 wave component. General correspodence for the main characteristic components in both sexes of Wistar rats and CBA mice has been confirmed. In two months diabetics a significant amplitude augmentation. (P8 wave component), but no change of latency of any early wave component, occurred. In diabetics with 11 months diabetes duration, highly significant reduction of P36 amplitude was observed. Moreover, latency prolongation on the late wave complex of SEPs (P30) in old diabetic rats might be seen as well. Conclusion: some comparative advantages of SEPs model in diabetic rodents for neuropathy studies were demonstrated. SEPs' good reproducibility and nonin-vasive approach for a longer period of time in the same animal, before and after diabetes therapy, allowed us to follow the onset and development of late diabetic complications.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA