Naslov
Enhanced expression of metallothioneins I/II in placenta and maternal liver, induced by syngeneic pregnancy and by peptidoglycan-monomer linked with zinc in mice

Autori
Jakovac, Hrvoje ; Grebić, Damir ; Mrakovčić-Šutić, Ines ; Rukavina, Daniel ; Radošević-Stašić, Biserka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
American Journal of Reproductive Immunology / Beaman, Kenneth - Singapur : Markano Print Media PTe Ltd, 2007, 465-465

Skup
X International Congress of Reproductive Immunology

Mjesto i datum
Opatija, Hrvatska, 10.06.2007. - 14.06.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
metallothioneins I/II; syngeneic pregnancy; peptidoglycan-monomer linked with zinc; placenta; liver

Sažetak
Problem: Liver regeneration that follows after partial hepatectomy (pHx) is a well-defined process, which involves the concerted action of numerous factors resulting in induction of cell replication and its inhibition at time when the entire liver mass is restored. Concomitantly, the breakdown of previously maintained tolerance and/or exposure to self antigens lead to the activation of natural immune repertoire, which through cytolytic activities participate in surveillance against aberrant cells and elimination of superfluous cells. Since in the induction of autoreactivity in these events a specific role might have gp96, acting as a chaperon and as a strong adjuvant, in this study we analysed its expression in regenerating liver, spleen and thymus, trying to correlate its presence with appearance of autorective clones of cells. Material and methods: Analyses were on early and late p.o. interval after 1/3 pHx or sham operation in C57Bl mice. Gp96, phenotype of lymphoid cells and apoptosis were detected by immunohistological staining, RT-PCR, FACScan and TUNEL analysis. Simultaneously in the thymus the cytotoxicity of intrahepatic and splenic mononuclear lymphatic cells (MNLC) obtained pHx donors were tested against syngeneic thymocytes and NK-sensitive targets (YAC-1) in wild, perforin and FasL deficient mice. Results: PHx induced upregulation of mRNA for gp96 in the regenerating liver, high cytoplasmic and nuclear over expression of gp96 staining in regenerating hepatocytes and a high surface expression of gp96 in the thymus. In the liver accumulated activated NKT cells (CD3intermediate/NK1.1+/CD69+cells and regulatory T cells (CD4+CD25+Foxp3+). Simultaneously hepatic MNLC became highly cytotoxic against syngeneic thymocytes, while later (at 24th and 48th hour) increased also the perforin and Fas/L-dependent cytotoxicity of hepatic and splenic MNLC against YAC-1 target. Conclusions: The data imply that during liver regeneration gp96 may serve as a natural adjuvant for chaperoning antigenic self peptides into the immune surveillance pathways, resulting in activation of autoreactive NKT cells, regulatory T cells and NK and LAK cells, which might be involved in reestablishment of previous morphostasis (Supported by grant 0621341-1337 from Croatian Ministry of Science).

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA