Naslov
Enhancing effects of syngeneic pregnancy and peptidoglycan-monomer linked with zinc on regulatory T cells in the maternal liver

Autori
Mrakovčić-Šutić, Ines ; Jakovac, Hrvoje ; Grebić, Damir ; Radošević-Stašić, Biserka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
American Journal of Reproductive Immunology / Beaman, Kenneth - Singapur : Markano Print Media PTe Ltd, 2007, 463-463

Skup
X International Congress of Reproductive Immunology

Mjesto i datum
Opatija, Hrvatska, 10.06.2007. - 14.06.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
syngeneic pregnancy; peptidoglycan monomer; regulatory T cells; maternal liver

Sažetak
Problem: Regulatory T cells (Treg) were claimed to be important players in the tolerance towards the fetus bearing alloantigens, having the pivotal role in inhibiting inappropriate activation of cell- and antibodies-mediated immune responses against self-antigens, as well as innate immune responses. They, however, might be related also with fetal invasion mechanisms at the maternal-fetal interface, as well as with the systemic hormonal changes, induced by pregnancy, showing that they reflect more global changes of the maternal immune system. Material and methods: In an attempt to enlarge our previous findings showing that during syngeneic pregnancy in the liver accumulate cytotoxic self-reactive NKT cells (Mrakovčić-Sutic et al. Scan. J. Immunol. 2003 ; 58:358-66), which usually cooperate with Treg cells, in this study we estimated the presence of CD4+CD25+Foxp3+ T cells in the liver, spleen and thymus of nonpregnant and pregnant C57/BL6 females matted with C57 BL/6 males. Phenotypic analysis and cytotoxicity against syngeneic thymocytes and YAC-1 targets was made on the 16th day after detection of vaginal plugs. Results: The data have shown that during syngeneic pregnancy in the maternal liver increases the proportion of CD4+CD25+Foxp3+, as well as CD3intermediate, NK1.1+ and IL-2Rb+ cells (NKT cells). Moreover, the treatment of mice with PGM-Zn additionally increased the Treg accumulation particularly in the liver. Furthermore, these findings correlated with the expression of heat shock protein gp96 in the liver and in the spleen.Conclusion: The data provide the new evidence about the interaction of NKT cells, that recognise self glycolipid antigens and immunosuppressive Treg cells and point to the liver as a place important for the activation of tolerance-promoting mechanisms during the pregnancy. (Supported by grant 0621341-1337 from Croatian Ministry of Science).

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA