Naslov
Formation of domains in multilamellar liposomes by sphingomyelin and cholesterol

Autori
Mirosavljević, Krunoslav ; Noethig-Laslo, Vesna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Prebiotic chemistry and early evolution / Noethig-Laslo, Vesna - Zagreb : Institut Ruđer Bošković, 2007

ISBN
978-953-6690-66-4

Skup
COST D 27 Prebiotic chemistry and early evolution

Mjesto i datum
Dubrovnik, Hrvatska, 11.05.2007. - 13.05.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
spin labelling; ESR; membrane model; phospholipids

Sažetak
In order to examine lipid domains formation in multilamellar liposomes due to the presence of sphingomyelin and cholesterol, liposomes composed of egg-phosphatidylcholine, sphingomyelin, cholesterol and dipalmitoylphosphatidylglycerol were prepared. Spin-labelled dipalmitoylphosphatidylcholine and palmitoyloleoylphosphatidylcholine were incorporated into liposomes. Spin label TEMPO was covalently attached to amino-group (instead of one methyl group). EPR parameters[1] from experimental and simulated spectra of the spin-labelled liposomes with different amount of cholesterol (0, 8, 15, 22, 29, 38 and 45 mol %) gave information about surface structure of the liposomes and lipid domain formation. Dynamics of the system was investigated by temperature dependence of the EPR spectra. Simulations of the spectra were performed by EasySpin software package[2] in the MatLab platform. [1] D. Marsh, Electron Spin Resonance (Membrane Spectroscopy), Springer-Verlag, Berlin Heidelberg, 1981, str. 51-142. [2] S. Stoll, A. Schweiger, J. Magn. Reson. 178 (2006) 42-55.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA