Naslov
Prevalence of Campylobacter spp. in broiler chickens in Croatia

Autori
Prukner-Radovčić, Estella ; Horvatek, Danijela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
World's Poultry Science Journal, XII European Poultry Conference, Book of Abstracts / Romboli, Isabella ; Flock, Dietmar ; Franchini, Achille - Barneveld : World Poultry Science Organisation, 2006, 112-113

ISBN
90-75980-329

Skup
XII European Poultry Conference

Mjesto i datum
Verona, Italija, 10.09.2006. - 14.09.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Campylobacter spp.; broiler chicken; multiplex PCR

Sažetak
Campylobacteriosis represent an important public health problem in Croatia, especially in children. Broilers are primarily colonized with C. jejuni, less often with C. coli and rarely with other species (Newel and Wagenaar, 2000). Poultry meat is a major source of campylobacteriosis and flock prevalence varies between EU countries, ranging from 5% to more than 90% (EU, 2003). Previous investigations regarding Campylobacter spp. in poultry in Croatia performed on feces of different poultry species revealed 11.69 % positive broiler samples (Krstulović et al., 2003). This investigation was conducted on selective media, and recently modern molecular diagnostic methods weren’ t used. To detect the prevalence of Campylobacter spp. in the northern part of Croatia, where the most of the broiler farms are located, cloacal swabs were randomly taken from 91 broiler chickens of different ages situated at three different farms. After pre-enrichment, cultures was streaked on modified charcoal cefoperazone desoxycholate agar (mCCDA) plates and incubated for 48 hours at 42&ordm ; C, in microaerobic atmosphere. All suspected colonies were presumptively identified as Campylobacter on the basis of macroscopic and microscopic appearance of colonies and positive catalase and oxidase reaction. The DNA was extracted from a 48 h cultures prepared in Nutrient Broth No. 2 as described by Jackson et al. (1993). The identification of strains was obtained by the multiplex PCR (mPCR) as described by Manfreda et al. (2003). The expected PCR amplicons were at 857, 589 and 462 by corresponding to the genus Campylobacter and to the species C. coli and C. jejuni, respectively. Altogether 35 (38.46%) samples were positive, whereas 18 (51.42%) of them were detected as C. coli and 17 (48.58%) as C. jejuni. Further investigation will be conducted on larger number of chickens to reveal the real prevalence of both Campylobacter strains. The mPCR, for the first time used in our laboratory, based on the use of different sets of primers is simple and time-saving method, can be performed for the simultaneous identification of different Campylobacter species and could contribute to the interpretation of results of epidemiological studies and determine whether particular Campylobacter types persist along the food chain or establish themselves as house flora in farms and slaughterhouses, what will be the goal of future investigations.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita, Veterinarska medicina

POVEZANOST RADA