Naslov
Seminal plasma and sperm bound lactate dehydrogenase LDH-C4 in infertile men

Autori
Gavella, Mirjana ; Cvitković, Petar ; Čolak, Božo

Izvornik
Diabetologia Croatica (0351-0042) 27 (1998), 1; 27-31

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
lactate dehydrogenase LDH-C4; seminal plasma; sperm bound; infertile men

Sažetak
Using our own modification of automated enzymatic assay for sperm-specific lactate dehydrogenase C4 activity, we simultaneously measured seminal plasma and sperm bound LDH C4 activities on an Abbott ABA-100 bichromatic spectrophotometer, using 2-oxohexanoate as substrate, with a precision of 5.2 percent. Both enzymatic activities were found to be significantly higher in oligozoospermic samples with sperm concentration below 20 million spermatozoa per ml of semen. The lactate dehydrogenase C4 activity/sperm ratio in seminal plasma was 17.4 in oligozoospermia vs. 8.1 in normozoospermia. Correlation analysis showed the seminal plasma LDH-C4 activity to be significantly related to the sperm-bound LDH C4 in normozoospermic men, whereas this relationship was less evident in oligozoospermic men. This data suggest the necessity of simultaneous determination of both sperm and seminal plasma LDH_C4 activities in oligozoospermic men to evaluate one of the possible causes of their infertility.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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