Naslov
Ganglioside expression in tissues of mice deficient in beta2-microglobulin

Autori
Markotić, Anita ; Marušić, Ana ; Jonjić, Stipan ; Mesarić, Marko ; Muething, Johannes

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual meeting of Croatian biochemists with international participation / - Zagreb, 1998

Skup
Annual meeting of Croatian biochemists with international participation

Mjesto i datum
Bizovac, Hrvatska, 17.09.1999. - 20.09.1999

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Sažetak
Aim. To investigate ganglioside expression in lymphoid (spleen, thymus, and lymph nodes) and other tissues (lungs, brain, muscle, liver) of mice deficient in beta2-microglobulin (B2MG), component of the histocompatibility class I antigens (MHC I). Methods. Gangliosides were isolated from C57BL/6 mice homozygous (-/-) or heterozygous (-/+) for the B2MG gene knockout and separated by high pereformance thin-layer chromatography (HPTLC). Individual gangliosides were visualized using resorcinol stain and HPTLC immunostainig overlay technique with polyclonal antibodies specific for GM3(Neu5Ac), GM3(Neu5Gc), GalNAcGM1b, and nLc4. GM1b-type gangliosides were detected after V. cholerae neuraminidase treatment with a specific anti-Gg4 antibody. Neuraminidase pretreatment was also used to detect gangliosides IV3nLc4 and VI3nLc6. GM1a and Gal-GalNac-GM1b gangliosides were detected with choleragenoid. Individual HPTLC bands were scanned with a CD60 scanner to quantify the differences between -/- and -/+ mice. Results. GM3(NeuAc) was four times and GM3(Neu5Gc) three times more abundant in the lungs of -/+ compared to -/- mice. Two bands of GM1b, GalNac-GM1b, and Gal- GalNac-GM1b, respectively, were expresses in the lungs of -/+ mice, but not in the lungs of -/- mice. Gangliosides of the neolacto-series were several times more abundant in the muscle, spleen, thymus, and lymph nodes of -/+ mice than of -/- mice. Conclusion. Our study provides in vivo evidence that B2MG is involved in the acyusition of distinct ganglioside profiles of different mouse organs.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA