Naslov
Protective effect of sialic acid in fructose induced human lens protein modification

Autori
Lipovac, Vaskresenija ; Gavella, Mirjana ; Ugrinović, Nikola ; Šverko, Višnja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts Annual Meeting of Croatian Biochemists / Glavaš-Orovac, Ljubica - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 1998, 90-90

Skup
Annual Meeting of Croatian Biochemists with international participation = Godišnji sastanak hrvatskih biokemičara

Mjesto i datum
Bizovac, Hrvatska, 17.09.1996. - 20.09.1996

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
sialic acid; fructose; human lens; protein

Sažetak
We investigated the effect of sialic acid, a keto hexose, on the formation of advanced glycation end product(AGE) in human lens protein, induced by in vitro glycation by fructose and glucose. Sialic acid inhibited fluorescence quenching by 15 percent. This probably reflect sialic acid transition metal chelation capacity, shown by 20 percent inhibition of Fe ferrozine formation. Sialic acid did not affect significantly neither the non-tryptophan novel fluorophore formation nor the tryptophan quenchinng induced by glucose. These results suggest that sialic acid exert its protective effect probably mainly through impeding fructose induced conformational changes of lens protein, and also in paert by its antioxidant action.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA