Naslov
Concentrations of TNF-alpha, CXCL8, big ET-1 and hsCRP in healthy non-smokers, smokers and COPD
(Concentrations of TNF-alpha, CXCL8, big ET-1 and hsCRP in healthy non-smokers, smokers and COPD patients)

Autori
Marević, Sanja ; Petrik, Jozsef ; Vrkić, Nada ; Popović-Grle, Sanja ; Čepelak, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Biochemia Medica, Abstracts of the 5th Croatian Congress of Medical Biochemists with international participation / Topić, Elizabeta ; Čvorišćec, Dubravka - Zagreb : Medicinska naklada, 2006, S141-S142

Skup
5th Croatian Congress of Medical Biochemists with international participation

Mjesto i datum
Poreč, Hrvatska, 18.10.2006. - 22.10.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
chronic obstructive pulmonary disease; TNF-alpha; big-ET-1; hsCRP

Sažetak
COPD (chronic obstructive pulmonary disease) is a chronic progressive inflammatory disease characterized by limitations in lung airflow that is not fully reversible. Although the pathophysiology of COPD is not understood completely, it is well known that various cells in the lungs are releasing inflammatory mediators. Tobacco smoke induces the release of a pro-inflammatory cytokine TNF-alpha (tumor necrosis factor-alfa) that might play a key role in COPD. TNF-alpha activates certain transcription factors and switches on the transcription of genes for cytokines, chemokines, proteases and endothelins. CXCL8 (interleukin-8) is a chemokine that attracts neutrophils to the inflammatory site. ET-1 (endothelin-1) and big ET-1 contribute to pulmonary vasoconstriction and pulmonary hypertension in COPD patients. hsCRP (highly sensitivity C-reactive protein) is an acute phase protein elevated in inflammatory diseases. The aim of this study was to investigate the concentrations and correlation of TNF-alpha, CXCL8, big ET-1 and hsCRP in healthy non-smokers, healthy smokers and patients with COPD. The concentration of TNF-alpha and CXCL8 in serum and big ET-1 in plasma was measured with an ELISA test in healthy non-smokers (n=23, control group), healthy smokers (n= 30) and COPD patients (n=30). hsCRP was measured in serum nephelometrically. The concentration of hsCRP was significantly higher in patients with COPD than in the control group (p=0.0004) but the concentrations of TNF-alpha (p=0.0788), CXCL8 (p=0.4839) and big ET-1 (p=0.3641) were not statistically different. There was no significant difference in the measured analytes between smokers and control group. Spearman coefficient of correlation between the concentrations of TNF- alpha and CXCL8 was r= 0.638 (P<0.0001). hsCRP was higher in COPD patients, which is in agreement with the fact that COPD is an inflammatory disease. hsCRP proved to be a more sensitive diagnostic parameter than TNF-alpha, CXCL8 and big ET-1 in the systemic circulation in patients with COPD.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Farmacija

POVEZANOST RADA