Naslov
Heat inactivation of human serum esterases hydrolysing phenylacetate

Autori
Reiner, Elsa ; Buntić, Anđelka ; Štuglin, Anita ; Barlović, Bojana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Godišnji sastanak hrvatskih biokemičara, Bizovačke Toplice, Sažeci znanstvenih priopćenja, ISBN 953-6256-28-2 / Glavaš-Obrovac, Ljubica - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 1998

Skup
Annual Meeting of Croatian Biochemists with International Participation

Mjesto i datum
Bizovac, Hrvatska, 17.09.1998. - 20.09.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
human serum esterases; phenylacetate; heat inactivation

Sažetak
The time course of heat inactivation of human serum esterases was studied with phenylacetate as substrate in 0.1 M Tris/HCl buffer pH=8.4 in the absence (total activity) and in the presence of 1.0 mM EDTA (EDTA-insensitive activity). Under these conditions the EDTA-insensitive activity at zero- time inactivation amounted to 2.2 % of the total activity. This in turn means that the total activity is almost completely due to the EDTA-sensitive enzyme(s). The EDTA-insensitive activity remained unaltered up to 30 min. Over the same time interval the EDTA-sensitive activity decreased, and the decrease followed the kinetics of the sum of two first order reactions ; about 80% activity decreased 30-times faster than the remaining 20% activity. This results indicates that human sera contain either two EDTA-sensitive esterases or one esterase in two conformational forms. In either case the EDTA-sensitive phenylacetate hydrolysis can be attributed to the arylesterase (EC 3.1.1.2).

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA