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Ћ Ћ ььЋ vj pmъHсmЦJ9 RЋ 3 Ј 0и Ћ ш‹ шЋ ььььЋ шьП tv:АрЋ ДD_vvve e ""фD• "" Immunoreactions caused by Hantaan and Andes viruses in MRC-5 and HEK293 cells Cebalo Lj1, Markoti A2 and Schmaljohn C3 1 Microbiology Service, Croatian National Institute of Publice Health, Zagreb, Croatia 2 Scientific Unit, University Hospital of Infectious Diseases  Dr. Fran Mihaljevi , Zagreb, Croatia 3 U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD, USA Hantaviruses (HTV), genus Hantavirus, family Bunyaviridae are rodent-borne viruses that are associated with two main clinical diseases: hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Little is known about the immunopathogenesis in HTV infections, and especially why different HTV cause mainly kidney disorders, while others mostly affect the lungs. The aim of this study was to analyze some non-specific imunoreactions regulated by two viruses; Hantaan virus (HFRS-causing) and Andes virus (HPS-causing) in two different cell types: human lung fibroblasts (MRC-5 cells) and human kidney cells (HEK293 cells). In lung fibroblasts we analyzed the gene expression for different cytokines/chemokines; extracellular matrix and adhesion molecules (ECM) and NF-kB signal transductional pathway in the early phase of infection (six hours). In HEK293 cells we analyzed the genes involved in different apoptosis pathways and changes in ECM gene expression in two time points during infection (3 and 5 days). To compare expression profiles of genes in RNA samples we used Focused Gene Expression cDNA Array Analysis (GEArrays, SuperArray Bioscience, Frederick, MD, USA). Selected genes identified by GEArray analysis were also analyzed by RT-PCR to verify transcriptional responses. 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