Naslov
Effect of caffeic acid phenethyl ester and caffeic acid, an antioxidant from propolis, on inducing apoptosis in HeLa human cervical carcinoma and Chinese hamster lung V79 fibroblast cells

Autori
Oršolić, Nada ; Bendelja, Krešo ; Brbot&#8211 ; Šaranović, Ana ; Bašić, Ivan

Izvornik
Periodicum biologorum (0031-5362) 106 (2004); 367-372

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
propolis; caffeic acid; caffeic acid phenethyl ester; apoptosis; necrosis; redox state

Sažetak
Background and purpose: Caffeic acid (CA) and caffeic acid phenethyl ester (CAPE) is phenolic antioxidants and active antitumor components of propolis. We aimed to determine the effects of CA, CAPE and water– soluble derivatives of propolis (WSDP) on colony growth, apoptosis, and redox state of HeLa human cervical carcinoma and Chinese hamster lung V79 fibroblast cells. Material and methods: WSDP, CA and CAPE were used in this studies and their cytotoxic effect on HeLa and V79 cells were studied. Colony formation assay and the annexin V method with detection of a hypodiploid cell population by flow cytometry were used for identification of cell growth and apoptosis, respectively. Results: CA and CAPE, but not WSDP, significantly suppressed colony growth. This effect was more pronounced against malignant HeLa cells than against normal V79 cells. CA and CAPE induced apoptosis of HeLa but not of V79 cells. Finally, glutathione levels in the V79 cells were unaffected by the tested compounds, whereas they were significantly reduced in HeLa cells. Conclusion: These results suggest that CA and CAPE preferentially affect the growth of malignant cells and that they can modulate the redox state of tumor cells, causing apoptosis.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA