Naslov
Direct and indirect mechanism(s) of antitumor activity of propolis and its polyphenolic compounds

Autori
Oršolić, Nada ; Brbot Šaranović, Ana ; Bašić, Ivan

Izvornik
Planta Medica (0032-0943) 72 (2006), 1; 20-27

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
water-soluble derivative of propolis; caffeic acid; caffeic acid phenethyl ester; mice; tumor growth; macrophages; nitric oxide; immunomodulation

Sažetak
The immunomodulatory action of a water-soluble derivative of propolis (WSDP) and two components of propolis, caffeic acid (CA) and caffeic acid phenethyl ester was investigated. Oral administration (50 mg/kg) of WSDP, CA, and CAPE enhanced weight and cellularity of spleen (p<0.05, p<0.01) of treated mice. The response of spleen cells to polyclonal mitogens (PHA, Con A, PWM) was also increased in mice treated with WSDP as compared to control (p<0.01) ; in contrast, the response of spleen cells of mice treated with CA were significantly suppressed (p<0.001). The colony forming ability of HeLa cells plated on monolayers of macrophages was completely inhibited by peritoneal macrophages from mice receiving either WSDP, CAPE, or CA. Macrophages from treated mice also inhibited  3H TdR incorporation into HeLa cells in vitro. Testing of the possible presence of NO in the supernatants of 24 hours cultured macrophages activated with either compound revealed that the toxicity of these cells to HeLa cells was in part due to the production of NO. Tumor growth was suppressed by WSDP and its polyphenolic compounds given orally to mice. Local presence of CA, and CAPE in the tissue caused significant delay of tumor formation. Based on these results, we postulate that the antitumor activity of test compounds includes pronounced immunomodulatory activity mainly due to the augmentation of non-specific antitumor resistance in mice via macrophage activation and production of soluble factors by those cells which may interfere with either cells of immune system or directly by tumor cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA