Naslov
DEVELOPMENTAL POTENTIAL OF MOUSE EMBRYOS CULTURED IN VITRO: A MORPHOLOGICAL ANALYSIS

Autori
Belovari, Tatjana ; Gajović, Srećko ; Kostović-Knežević, Ljiljana

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
2nd Croatian Congress of Croatian Society for Electron Microscopy with International Participation / Srećko Gajović - Zagreb, 2006, 150-151

Skup
2nd Croatian Congress of Croatian Society for Electron Microscopy with International Participation

Mjesto i datum
Topusko, Hrvatska, 18.05.2006. - 21.05.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
embryo culture; histology; mouse

Sažetak
The long term stationary culture of postimplantation embryos without extraembryonic membranes is a method to assess their development in vitro (1). When gastrulating rat embryos are cultivated, epidermis, cartilage, gut and respiratory epithelium, neuroblasts and myotubes develop and reach a high degree of differentiation (2, 3). In order to determine the degree of tissue differentiation in cultivated mouse embryos, electron microscopy was used to analyse their ultrastructural characteristics. Gastrulating CD1 mouse embryos were cultivated in Dulbeco’ s Modified Eagle Medium with 20% fetal bovine serum for 14 days and compared with cultivated gastrulating Fisher rat embryos. After 14 days in vitro culture, various tissues developed: different epithelia, neuroblasts, connective tissue, cartilage, blood vessels and myotubes (Fig. 1). Electron microscopy showed a high extent of ultrastructural differentiation of obtained tissues (Fig. 2). It can be concluded that mouse embryos after long term culture can reach a high degree of differentiation which was comparable to that observed in rats.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA