Naslov
IL-18 and TNF-α promoter polymorphisms and susceptibility to type 1 diabetes in the Dalmatian population

Autori
Boraska, Vesna ; Bečić, Kristijan ; Škrabić, Veselin ; Peruzović, Marijana ; Čikeš Čulić, Vedrana ; Kapitanović, Sanja ; Zemunik, Tatijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European Human Genetics Conference 2006 : abstracts / European Society of Human Genetics - Amsterdam : Nature publishing group, 2006, 332-332

Skup
European Human Genetics Conference 2006

Mjesto i datum
Amsterdam, Nizozemska, 06.05.2006. - 09.05.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
IL-18; TNF-alpha; type 1 diabetes; Dalmatia

Sažetak
Type 1 diabetes mellitus (T1DM) is characterized by a chronic progressive inflammatory autoimmune reaction that causes the selective elimination of pancreatic β cells. Proinflammatory cytokines, interleukin-18 (IL-18) and tumor necrosis factor alpha (TNF-α ), both have been implicated in the pathogenesis of T1DM. Additionally IL-18 plays a role in regulation of TNF-α production. Therefore we tested an association of two single nucleotide polymorphisms (SNP) in promoter regions of these genes with the susceptibility to T1DM in the Dalmation population (South Croatia). 134 T1DM patients and 132 control subjects were tested for a G-137C change in a promoter region of IL-18 gene by a sequence specific PCR. TNF-α G-308A promoter variant was genotyped in a larger group of T1DM patients (206) and controls (144) by standard PCR followed with NcoI endonuclease restriction. Data were analysed using chi-square test. A distribution of IL-18 G-137C and TNF-α G-308A promoter variants was equal between the T1DM patients and the controls (p=0.9448 and p=0.0655, respectively). The present study provided no evidence of association of IL-18 and TNF-α promoter variants with susceptibility to T1DM in Dalmatian population. However as genotype frequencies for TNF-α G-308A polymorphism were close to the limit of statistical significance (p=0.0655) further analysis should be conducted.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

Napomena
European Journal of Human Genetics (ISSN 1018-4813)

POVEZANOST RADA