Naslov
Gender-dependent expression of rat renal organic anion transporter OAT5 is caused by androgen inhibition

Autori
Ljubojević, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
Gottinger Transporttage 2006

Mjesto i datum
Göttingen, Njemačka, 04.11.2006. - 05.11.2006

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
immunocytochemistry; mammalian kidney; sex differences; sex hormones; protein expression

Sažetak
We have re-investigated the expression of rat organic anion transporter 5 (rOat5) mRNA by RT-PCR in various kidney tissue zones and localization of its protein by Western blotting (WB) of isolated cell membranes and by immunocytochemistry (IC) in tissue cryosections from adult intact and gonadectomized male (M) and female (F) rats, castrated M rats treated with various sex hormones, and prepubertal intact M and F rats. In adult rats, the expression of rOat5 mRNA was predominant in the outer stripe, where it exhibited: a) gender differences (F>M), b) upregulation in M and downregulation in F by gonadectomy, and c) strong downregulation in testosterone- and slight upregulation in estradiol- and progesterone-treated castrated M. The WB data in isolated kidney membranes from variously-treated rats, and the IC pattern of brush-border staining intensity in PT S3 segments in the outer stripe matched the mRNA data. In prepubertal rats, the expression of rOat5 protein in PT was weak and similar in M and F, thus indicating that the observed gender differences in the expression of rOAT5 appear after puberty due to strong androgen inhibition and weak estrogen and progesterone stimulation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA