Naslov
Overexpression of protein p16INK4a as a marker for cervical dysplasia induced by HPV infection.

Autori
Krivak Bolanca I, Radić Vanja, Ciglar S.

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Update on Human Papillomavirus Infection and Cervical Pathology / Monsonego J. - Bolonja : Medimond International Proceedings, 2006, 25-30

Skup
Eurogin 2006. 6thInternational multidisciplinary Congress

Mjesto i datum
Pariz, Francuska, 23.04.2006. - 26.04.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
karcinom cerviksa; prekanceroza; tumorski marker; p16INK4a
(cervical carcinoma; precancerosis; tumor marker; p16INK4a)

Sažetak
OBJECTIVE: Success in diagnoses of cervical carcinoma is due to the population-wide screening protocols which allow identifying early precancerogenic lesions by the use of cytological smear analysis. Based on the understanding of the molecular pathogenesis of cervical cancer and the key role of the human papilloma virus oncogenes, the cyclin-dependent kinase inhibitor p16INK4a emerged as an biomarker to identify HPV-infected cells in histological and cytological slides. Protein p16INK4a was named as a diagnostic marker for cervical dysplasia.PURPOSE OF THE STUDY was to exam the potential of p16 INK4a as a biomarker for oncogenic HPV infection in cervical lesions.MATERIAL AND METHODS: Conventional Pap-smears were taken from 79 patients, analyzed and quategorisated by Bethesda into normal epithelium (14 patients), low grade (LG SIL)(24), high grade lesion (HG SIL)(31) and squamous carcinoma (10). Simultaneously, fifty-two (52) slides were taken for immunoassaying with p16INK4a and twenty-seven (27) were decolorized in diluted solution of HCl, rinsed with distilled water and immunocytochemically stained. High risk HPV infection was documented in all high grade and ca patients (100%), and in 15 patients with low grade lesion (62, 5%). Forty-one patients underwent colposcopy and/or operation so histological diagnoses were made.RESULTS: Cells are regarded as stained in which p16INK4a was expressed in nuclei and/or in a cytoplasm.There was sporadic positivity in, mainly , metaplastic cells but only in their cytoplasms. The portion of p16INK4a-positive samples increases in the following row: normal (1/14 - 7, 14%) - LG SIL (7/24 - 29, 16%) - HG SIL (100%) – carcinoma (100%). CONCLUSION: In spite of sporadic positivity of normal metaplastic cells use of p16INK4a may be regarded as a supplementary test for diagnostics of cervical dysplasia. Overexpression of the protein p16INK4a is typical for lesions where the persistent HPV infection exist (neoplastic and high grade lesion), although it seemed that another trigger is necessary for LG lesion so cells may switch into a process of carcinogenesis.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA