Naslov
COMPARISON BETWEEN THE FREQUENCIES OF STABLE AND UNSTABLE CHROMOSOMAL ABERRATIONS IN GENERAL POPULATION OF THE REPUBLIC CROATIA

Autori
Želježic, Davor ; Fučić, Aleksandra ; Kopjar, Nevenka ; Kašuba, Vilena ; Rozgaj, Ruzica ; Ramić, Snježana ; Lucas, Joe Nathan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Programme and Abstracts: From Genes to Molecular Epidemiology / - Prag, 2006

Skup
36th Annual Meeting of the European Environmental Mutagen Society

Mjesto i datum
Prag, Češka Republika, 02.07.2006. - 06.07.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
FISH; control subjects; translocations; chromosome painting

Sažetak
Fluorescence in-situ hybridization (FISH) using whole chromosome paints is a potent tool in retrospective biological dosimetry. Generally regarded as a ‘ gold standard’ , three-color FISH is used in studies for comparison of translocation frequency with chromosome aberrations detectible by conventional cytogenetics. The aim of our work was to determine the baseline translocation yield in the peripheral blood lymphocytes using whole-chromosome paint probes for chromosomes 1, 2 and 4, and to compare it with the results of the standard chromosomal aberration analysis. The study comprised 20 individuals from the general population of the Republic Croatia (10 males and 10 females). Both gender groups were of the similar age. The mean age of the male group was 47 years (range 37 - 58), and the female group 41 (range 31 – 50). For each subject more than 1000 genome equivalents were analysed. Translocation frequencies were calculated according to Lucas et al. (1992). The translocation and chromosomal aberrations per 100 cells for male and female subjects were not significantly different, 0.41 for males and 0.3 for females. In lymphocytes of all subjects, chromatid and chromosome breaks and acentric fragments detected were in concordance with historical background values for this age group. The percentage of chromatid breaks found in males was 0.14, and in females 0.10. In females 0.1 % of chromosome breaks (0.1 % in males) and 0.07 % of acentrics (0.02 % in males) was found. Detected translocation frequency is in agreement with historical values for the analysed age group. Due to the limited number of European biodosimetry centres, the launching of common databank and standardization of selection of chromosomes for genome equivalent calculations is of great significance.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA