Pregled bibliografske jedinice broj: 266748
The protective effect of interleukin 1alpha and IL-1beta on the acetaminophen induced liver toxicity relies on the early induction of their endogenous expression by acetaminophen itself
The protective effect of interleukin 1alpha and IL-1beta on the acetaminophen induced liver toxicity relies on the early induction of their endogenous expression by acetaminophen itself // 6th International Cytokine Conference 2006 Vienna, Austria. European Cytokine Network 1, (Suppl 2006), str.44. / Schwartzmeier Joseph (ur.).
Beč: Medimond International Proceedings, 2006. str. 1-44 (poster, međunarodna recenzija, sažetak, znanstveni)
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Naslov
The protective effect of interleukin 1alpha and IL-1beta on the acetaminophen induced liver toxicity relies on the early induction of their endogenous expression by acetaminophen itself
Autori
Aleksić, Joško ; Čulo, Melanie-Ivana ; Poljak, Ljiljana ; Matić, Tomas ; Čulo, Filip
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
6th International Cytokine Conference 2006 Vienna, Austria. European Cytokine Network 1, (Suppl 2006), str.44.
/ Schwartzmeier Joseph - Beč : Medimond International Proceedings, 2006, 1-44
Skup
6th International Cytokine Conference 2006
Mjesto i datum
Beč, Austrija, 27.08.2006. - 31.08.2006
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
interleukin 1beta and IL-1alpha; IL-1Ra; acetaminophen; liver toxicity
Sažetak
It is known that inflammatory cytokines influence the effect of hepatotoxic chemicals on liver. Previously we have shown that IL-1alpha and IL-6 have hepatoprotective effect if given to mice before administration of acetaminophen (APAP) and that this effect is partially mediated by PGE2. In these experiments we investigated the influence of IL-1beta in the same model, since we have been able to modulate its effect with specific antagonists (IL-1Ra and polyclonal anti- IL-1beta antibodies). APAP (300 mg/kg for survival and 200 mg/kg for determination of aminotransferases) or saline in controls were administered intragastrically to mice which were given phenobarbitone in drinking water for 7 days and fasted overnight IL-1beta 500 IU /mouse) or IL-1beta antibody (0.5 ml of rabbit to mouse IL-1, obtained by three consecutive injection of IL-1beta , first in complete FCA) were given i.p. 3 hours before APAP and IL-Ra (2.5 mg/mouse) half hour before APAP. The survival of mice was followed for 72 hours and concentration aminotransferases (AST and ALT) serum were determined 18-24 hours after administration of APAP. IL-1beta significantly increased the survival mice and decreased serum level of AST and ALT (p < 0.05). Just opposite effect had IL-1beta antibodies, but the effects were not significant. Similarly IL-1Ra, significantly increased serum concentration of AST and ALT (p < 0.05 or better) (the survival was not tested in this model). The finding that that IL-1Ra alone raised serum level of aminotranferases as well that potency IL-1beta antibodies alone showed the tendency to worse the manifestations of intoxication, promoted us to analyze the synthesis if IL-1 cytokines in non-treated animals intoxicated with AAP. RT-PCR analysis of the IL-1beta and IL-1alpha expression level in liver samples from APAP intoxicated mice has revealed that acetaminophen induces the expression of both cytokines already 1 hour after its intragastric administration. This expression was even higher at 6 hours following APAP administration and could be blocked by intravenous administration of aspirin at the dose which inhibits the activity of NF-kappa B transcription factor. Based on these results we hypothesized that organism intoxicated with APAP synthesizes its own IL-1 early on following APAP administration, which would represent a host defense reaction that could be helped by applying appropriate dose of exogenous cytokines.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
108-0000000-0328 - Uloga proupalnih citokina i prostaglandina u akutnom oštećenju jetre
Ustanove:
Medicinski fakultet, Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE