Pregled bibliografske jedinice broj: 265227
Cholinesterase activity assays: Molar absorption coefficient of the reduced Ellman reagent
Cholinesterase activity assays: Molar absorption coefficient of the reduced Ellman reagent // The Proceedings of the Fourth Chemical and Biological Medical Treatment Symposium (CBMTS IV) / Portmann, Rudolf (ur.).
Aberdeen (MD): Richard Price, Applied Science and Analysis, 2003. str. 267-270 (poster, međunarodna recenzija, cjeloviti rad (in extenso), znanstveni)
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Naslov
Cholinesterase activity assays: Molar absorption coefficient of the reduced Ellman reagent
Autori
Reiner, Elsa ; Šinko, Goran ; Štuglin, Anita ; Simeon-Rudolf, Vera ; Worek, Franz ; Kiderlen, Daniela ; Eyer, Peter
Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni
Izvornik
The Proceedings of the Fourth Chemical and Biological Medical Treatment Symposium (CBMTS IV)
/ Portmann, Rudolf - Aberdeen (MD) : Richard Price, Applied Science and Analysis, 2003, 267-270
Skup
CB Medical Treatment Symposium, An Exploration of Present Capabilities and Future Requirements
Mjesto i datum
Spiez, Švicarska, 28.04.2002. - 03.05.2002
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
cholinesterase; activity assays; molar absorption coefficient; Ellman reagent
Sažetak
For the calculation of cholinesterase activities measured by the Ellman spectrophotometric method (in phosphate buffer pH=7.4) using thiocholine ester substrates, the following molar absorption coefficients (ε M) for the reduced Ellman reagent are suggested: 14.1 x 103 and 13.8 x 103 M-1cm-1 for activities measured at 412 nm and 25 or 37 ˚ C resp., and 10.8 x 103 and 11.0 x 103 M-1cm-1 for activities measured at 436 nm and 25 or 37 ˚ C resp.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
POVEZANOST RADA
Projekti:
0022014
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb