Naslov
Determination of membrane integrity in dogs sperm

Autori
Dobranić, Tomislav ; Samardžija, Marko ; Cergolj, Marijan ; Prvanović, Nikica

Izvornik
Veterinarski arhiv (0372-5480) 75 (2005), 1; 23-30

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
canine semen ; progressive motility ; HOS-test ; membrane integrity

Sažetak
In our research we used semen samples from 9 sexually mature dogs from different breeds, in age from one till five years. Quality of sperm we evaluated using next parameters: progressive motility (subjective), than structural and functional integrity of sperm membrane (supravital staining (eosine/nygrosine) and HOS test). The majority of HOS reactive or swelled up spermatozoa we got in hypoosmolar solution of 100 mosmol by incubation of 30 minutes. Results of research are elaborated by statistical method ANOVA and Tukey HSD tests post-hoc analysis and we also determined correlations between semen parameters. Between middle values for HOS reactive (swollen up) spermatozoa in hypoosmolar solutions with different osmolality we determined significant differences (P<0, 0001). Using hypoosmotic solution with osmolality 100 mosmol, we determined significantly strong positive correlations between HOS test and progressive motility (r=0, 82 ; P<0, 05), between HOS test and supravital staining eosine/nygrosine (r=0, 98 ; P<0, 05) and between progressive motility and supravital staining eosine/nygrosine (r=0, 89 ; P<0, 05). It is expected because sperm motility partially depends of functional integrity of sperm membrane and other events included in metabolism of the sperm. Results of our research have shown that HOS test can be easy method for routine evaluation of sperm quality in dogs and also has very useful role in determination of HOS reactive and progressive motile spermatozoa and spermatozoa with structurally intact plasma membrane.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA