Naslov
Protective Effect of Propolis and Flavonoids on Radiation-induced Chromosomal Damage

Autori
Horvat-Knežević, Anica ; Benković, Vesna ; Kopjar, Nevenka ; Ramić, Snježana ; Lisičić, Duje ; Oršolić, Nada ; Bašić, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
9. hrvatski biološki kongres s međunarodnim sudjelovanjem : Zbornik sažetaka = 9th Croatian Biological Congress with International Participation : Proceeding of abstracts / Besendorfer, Višnja ; Klobučar, Goran I.V. - Zagreb : Hrvatsko prirodoslovno društvo, 2006, 99-101

ISBN
953-6241-06-4

Skup
Hrvatski biološki kongres s međunarodnim sudjelovanjem (9 ; 2006)

Mjesto i datum
Rovinj, Hrvatska, 24.03.2006. - 29.03.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
propolis; makrofage activity; tumor; immunomodulation

Sažetak
DNA damage induced by ionizing radiation is mediated through free radical formation. The radioprotective effects of propolis and flavonoids were investigated by using the cytochalasin-B blocked micronucleus test, and frequency of micronucleus (MN) was scored in binucleacted cells. The nuclear proliferation index was calculated. A single administration of water-soluble derivate of propolis (WSDP), hydroalcoholic solution of propolis (HASP) in doses of 100 mg/kg, and quercetin, caffeic acid, chrysin and naringin in dose of 50 µ ; ; M were used 30 minutes before irradiation. Irradiation was performed using a 60Co γ -ray source, and absorbed dose was 4 Gy. Treatment with HASP, caffeic acid, chrysin and naringin significantly increase MN frequency, while treatment with WSDP and quercetin decrease MN frequency in irradiated samples. In nonirradiated samples administration of HASP significantly increase MN frequency. Nuclear proliferation index (NDI) was increased in non-irradiated samples treated with all tested compounds. WSDP, chrysin and naringin significantly decreased, while quercetin significantly increased the value of NDI. The resultes of this investigation get us a better insigth of potential use of propolis and investigated flavonoids as radioprotectors.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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