Naslov
The selective effect of propolis and its polyphenolic compounds on primary culture of human urinary bladder cancer and normal epithelial cells

Autori
Oršolić, Nada ; Štajcer, Damir ; Šver, Lidija ; Kosalec, Ivan ; Bakmaz, Marina ; Terzić, Svjetlana ; Bašić, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
9. hrvatski biološki kongres s međunarodnim sudjelovanjem : Zbornik sažetaka = 9th Croatian Biological Congress with International Participation : Proceeding of abstracts / Besendorfer, Višnja ; Klobučar, Goran I. V. - Zagreb : Hrvatsko biološko društvo, 2006, 244-246

ISBN
953-6241-06-4

Skup
Hrvatski biološki kongres s međunarodnim sudjelovanjem (9 ; 2006)

Mjesto i datum
Rovinj, Hrvatska, 23.09.2006. - 29.09.2006

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
propolis; tumor; mokraćni mjehur
(propolis; tumor; urinary bladder)

Sažetak
Large amounts of epidemiological data have supported the inverse relation between the consumption of fruits and vegetables and the incidence of cancer. The flavonoids present in propolis are considered to be rich source of chemopreventive agents, since they have various therapeutic biological activities. This study was carried out to find whether propolis and its polyphenolic/flavonoids compounds may induce cytotoxicity in primary culture of human urinary bladder cancer cells as compared to normal urinary bladder epithelial cells. Pieces of urinary bladder cancer grown in the cavity of urinary bladder as well as normal epithelial tissue from urinary bladder wall were taken from patients in different stages (grade G1, G2, G3) of bladder carcinoma. Single cells suspension from pieces of either cancer or normal tissue was prepared according standard laboratory procedure. Incubation for cytotoxicity testing were carried out in RPMI-1640 medium with 20% FCS at 37 ˚ C with 5% CO2 with or without different concentration (50, 150, 300 µ ; ; g/ml) of test components. The cytotoxicity of two preparations of propolis (water and ethanolic extract of propolis ; WSDP or EEP) and its polyphenolic compounds (caffeic acid, naringin, chrysin, and quercetin) was determined using trypan blue exlusion assay. These findings suggest that EEP is the most effective in inhibition of urinary bladder cancer cell proliferation as compared to flavonoids derived from propolis. All test components have no cytotoxic effect on normal epithelial cells. The result of this study may provide great impact on the potential activity of EEP as an adjuvant to surgery, to suppress or prevent tumor recurrence in urinary bladder since only a few anti-cancer drugs have been effective in tumor control. Since immunomodulation by BCG has been used to improve the results of surgery it is likely that propolis preparation (EEP) as immunomodulating compound may be a substitute for mycobacterial treatment since propolis preparation have expressed no side effect after treatment. However, the exact cancer chemoprevention mechanisms of propolis effectiveness have to be elucidated.

Izvorni jezik
Hrvatski, engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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