Naslov
Xenobiotic clenbuterol: Toxicologic implication of persisting residues in liver as edible tissue

Autori
Gojmerac, Tihomira ; Pleadin, Jelka ; Lipej, Zoran ; Novosel, Dinko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 43rd Congress of the European Societies of Toxicology : EUROTOX 2006/6 CTDC Congress ; u: Toxicology Letters 164 (2006) (S1) P22-19 / - , 2006, S277-S278

Skup
43rd Congress of the European Societies of Toxicology (43 ; 2006) ; Congress of Toxicology in Developing Countries (6 ; 2006)

Mjesto i datum
Cavtat, Hrvatska, 20.09.2006. - 24.09.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
clenbuterol; toxicology; residues; liver

Sažetak
The aim of the present study was to evaluate the clenbuterol residue depletion in pig liver as edible tissue on days 0, 3, 7, 14 and 35 of treatment discontinuation, in correlation with the severity of histopathologic changes at the same time points. A growth-promotion dose of clenbuterol (20 μ g/kg body mass per day) was administered orally to pigs for 28 days. Upon cessation of administration, on days 0, 3, 7, 14 and 35, the pigs were randomly sacrificed and the liver was collected for residual clenbuterol determination and histopathologic examination. Based on analytical assay procedure utilizing validated enzyme-linked immunosorbent assay, clenbuterol residues in pig liver declined from 30.19± ; 17.70 ng/g, 7.01± ; 1.37 ng/g, 1.32± ; 0.88 ng/g and 0.40± ; 0.11 ng/g on days 0, 3, 7 and 14, respectively, to 0.22± ; 0.04 ng/g on day 35. On day 14, the measured clenbuterol residues fell below the maximal residue level (MRL) of 0.5 ng/g, however, quantification of clenbuterol residues indicated persistence of this β 2-adrenergic agonist in the liver as edible tissue on day 35 of treatment cessation. Histopathologic studies performed at the same time points revealed vacuolar hepatocyte degeneration, with glycogen depletion from the cytoplasm. The severity of degenerative changes of the liver tissue did not follow clenbuterol depletion upon treatment cessation but remained unchanged, suggesting an irreversible effect of clenbuterol residues during the study period.

Izvorni jezik
Engleski

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