Pregled bibliografske jedinice broj: 257736
Characterization of an atrazine-degrading community enriched from agrochemical factory soil
Characterization of an atrazine-degrading community enriched from agrochemical factory soil // Integrating Microbial Knowledge into Human Life. 2nd FEMS Congress of European Microbiologist / Federation of European Microbiological Societes (ur.).
Madrid, 2006. str. 183-183 (poster, međunarodna recenzija, sažetak, znanstveni)
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Naslov
Characterization of an atrazine-degrading community enriched from agrochemical factory soil
Autori
Udiković Kolić, Nikolina ; Hršak, Dubravka ; Martin-Laurent Fabrice
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Integrating Microbial Knowledge into Human Life. 2nd FEMS Congress of European Microbiologist
/ Federation of European Microbiological Societes - Madrid, 2006, 183-183
Skup
2nd FEMS Congress of European Microbiologists
Mjesto i datum
Madrid, Španjolska, 04.07.2006. - 08.07.2006
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
atrazine; biodegradation
Sažetak
An atrazine degrading bacterial community was enriched from agrochemical factory soil exposed to repeated spills of effluent from atrazine synthesis. The community consisted of at least four strains, possessing catabolic activities and mineralized 78.4% of (14C-ring) atrazine as well as 35.6% of (ethyl-14C) atrazine within 6 days batch cultivation in mineral salts medium. Atrazine-degrading genetic potential of the community estimated by PCR revealed the presence of trzN, atzB, atzC and trzD genes. Based on partial 16S rDNA sequencing, two strains (ATZ1 and ATZ2) showed close phylogenic relationships to Arthrobacter crystallopoietes (99%) and Arthrobacter keyseri (98%), respectively. Further study of catabolic activities showed that strains ATZ1 and ATZ2 were capable of dechlorinating atrazine and contained the trzN. The strain ATZ2 continued to degrade hydroxyatrazine to cyanuric acid and contained atzB and atzC, while the strain ATZ1 accumulated hydroxyatrazine and contained atzC. Two other community members, tentatively identified as Achromobacter species, contained trzD known to be responsible for cyanuric acid degradation, which suggested their important role in completing atrazine degradation. The obtained results also suggested that in addition to the combined metabolic activities a different type of interactions may exist between community members during atrazine mineralization.
Izvorni jezik
Engleski
Znanstvena područja
Biologija