Pregled bibliografske jedinice broj: 256337
Cytoskeleton alterations in cisplatin-resistant cells: The role of Rho GTPases in cisplatin toxicity
Cytoskeleton alterations in cisplatin-resistant cells: The role of Rho GTPases in cisplatin toxicity // FEBS/EMBO Workshop 15th Protein Kinase Meeting „ ; Spatial and Temporal Regulation of Signalling“ ; / FEBS/EMBO (ur.).
Oslo: Federation of European Biochemical Societies (FEBS), 2006. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 256337 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Cytoskeleton alterations in cisplatin-resistant cells: The role of Rho GTPases in cisplatin toxicity
Autori
Čimbora-Zovko, Tamara ; Ljubojević, Marija ; Sabolić, Ivan ; Fritz, Gerhard ; Osmak, Maja
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
FEBS/EMBO Workshop 15th Protein Kinase Meeting „ ; Spatial and Temporal Regulation of Signalling“ ;
/ FEBS/EMBO - Oslo : Federation of European Biochemical Societies (FEBS), 2006
Skup
FEBS/EMBO Workshop 15th Protein Kinase Meeting „ ; Spatial and Temporal Regulation of Signalling“ ;
Mjesto i datum
Oslo, Norveška, 21.09.2006. - 24.09.2006
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
cisplatin; cytoskeleton; Rho GTPase
Sažetak
Acquired resistance to cisplatin represents a major obstacle to successful chemotherapy. We have developed cisplatin-resistant CA3ST cells, which exhibited pronounced stress fibers and numerous focal contacts, in contrast to parental human laryngeal carcinoma HEp-2 cells that displayed cortical actin organized in adhesion belt and few vinculin-containing focal adhesions. In line with these cytoskeleton alterations, CA3ST cells had increased expression of RhoA GTPase, on both protein and mRNA level, while the expression of Rac1 was unchanged comparing to HEp-2 cells. A single cisplatin treatment of HEp-2 cells induced formation of stress fibers and vinculin relocalization to focal contacts, which was preceded by translocation of RhoA, but not Rac1, to the cell membrane. Pretreatment with lovastatin decreased the level of membrane-bound RhoA, leading to increased fraction of apoptotic cells after cisplatin treatment. Moreover, since CA3ST cells were more sensitive than HEp-2 cells to lovastatin-induced apoptosis, lovastatin pretreatment restored sensitivity of resistant cells to cisplatin to the level found in parental cells. In addition, cisplatin-resistant human cervical carcinoma cells also showed increased RhoA protein and mRNA. Therefore, we speculate that upregulation of RhoA GTPase could be a general phenomena accompanying cisplatin resistance and might be involved in cellular response to cisplatin.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Institut "Ruđer Bošković", Zagreb
Profili:
Marija Ljubojević
(autor)
