Naslov
JAK-1/Smad signaling dependent genes expression associated with neurogenic to astrogliogenic transition point of brain development

Autori
Slade, Neda ; Zorić, Arijana ; Horvat, Branka ; Vukšić, Mario ; Kostović, Ivica ; Poljak, Ljiljana

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
The Proceedings of the 6th Joint Meeting of the International Cytokines Society (ICS), International Society of Interferon and Cytokine Research (ISICR) and the European Cytokine Society (ECS) / Schwarzmeier, Josef - Bolonja : Medimond International Proceedings, 2006, 1-5

ISBN
88-7587-279-1

Skup
6th Joint Meeting of the International Cytokines Society (6 ; 2006) ; International Society of Interferon and Cytokine Research ; the European Cytokine Society

Mjesto i datum
Beč, Austrija, 27.08.2006. - 31.08.2006

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cytokine signaling; brain development; gliosis; brain injuries

Sažetak
To address the question on the role of a Jak-1 molecule in the TGF-beta controlled sequential process of brain development, we have used cell lines either deficient or enriched in Jak-1. Upon their transfection with TGF-beta receptor associated molecules - Flagged Smad-3, -2 or -1, the expression pattern of Neurogenin 1 and Glial Fibrillary Acidic Protein (GFAP) related to NF-kB activation has been followed. We found that no expression of Neurogenin 1 or GFAP can be induced in mutant cells following single transfection. However, based on the p65 nuclear translocation, we have found that NF-kB transcription factor becomes activated following transfection with the plasmid encoding Smad1 which plays a central role in the alternative TGF-beta signaling triggered via ALK-1 receptor. Decreased Smad-1 mRNA expression in the same cells upon transfection with plasmid encoding Smad-2, indicates the Jak-1/Smad2 dependent signaling likely interfere with ALK-1 mediated pathway to which a neuroprotective role has been assigned.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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