Naslov
Lipid-Protein Interactions in Human Plasma LDL Evidenced by Magnetic Resonance

Autori
Kveder, Marina ; Marinić, Željko ; Kriško, Anita ; Vikić-Topić, Dražen ; Pifat, Greta

Izvornik
Chemistry and Physics of Lipids (0009-3084) 141 (2006), 1-2; 225-229

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Lipid– protein interaction ; apoB ; Relaxation enhancement

Sažetak
Low density lipoprotein (LDL) particles exhibit extremely complex three-dimensional structural organization which is still not understood at the molecular level. The aim of this study was to provide the experimental evidence of a direct non-covalent interaction of the protein part with the lipid matrix. The approach was based on the combination of 1H NMR (600 MHz) spectroscopy with thiol-specific spin labeling of the protein (apoB). It is shown that the spectral peaks assigned to the methyl head groups of phosphatidylcholine and sphingomyelin in the 1H spectra of LDL exhibit line broadening when otherwise free thiol groups of apoB are covalently modified by methanethiosulfonate spin label. The effect is similar in the presence of water soluble paramagnetic compound. These results indicate that fragments of apoB, which are part of the receptor binding region, are directly in contact with the solvated phospholipid head groups of the lipid matrix.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA