Pregled bibliografske jedinice broj: 255148
Differential transduction efficacy of adenoviruses containing NGR within cyclic and linear sequences in HI loop of fiber protein to aminopeptidase N and alpha V beta 3 integrins
Differential transduction efficacy of adenoviruses containing NGR within cyclic and linear sequences in HI loop of fiber protein to aminopeptidase N and alpha V beta 3 integrins // Bioscience 2006: Programme and abstracts
Glasgow, 2006. (poster, nije recenziran, sažetak, znanstveni)
CROSBI ID: 255148 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Differential transduction efficacy of adenoviruses containing NGR within cyclic and linear sequences in HI loop of fiber protein to aminopeptidase N and alpha V beta 3 integrins
Autori
Majhen, Dragomira ; Gabrilovac, Jelka ; Richardson, Jennifer ; Eloit, Marc ; Ambriović-Ristov, Andreja
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Bioscience 2006: Programme and abstracts
/ - Glasgow, 2006
Skup
Bioscience 2006-bioscience for the 21st century
Mjesto i datum
Glasgow, Ujedinjeno Kraljevstvo, 23.07.2006. - 27.07.2006
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
adenovirus; gene retargeting; aminopeptidase n
Sažetak
The aim of this work was to design an gene therapy applicable Ad vector retargeted on the APN molecule expressed on angiogenetic cells, via insertion of specific NGR motiv (asparagine-glycine-arginine) into the HI loop of a fiber protein. On the rhabdomyosarcoma (RD) cell line, which expresses APN but only very low levels of α vβ 3 integrin and CAR receptor, all our NGR-bearing Ad exhibited moderately increased transduction efficacy in comparison to wild type virus. NGR-bearing Ad containing cyclic motifs were more efficient than those containing linear ones. The increased transduction efficacy of NGR-bearing Ads was completely abolished by the APN-specific peptide CNGRC and the integrin-specific peptide CRGDC. By measuring transduction efficacy on human laryngeal carcinoma cells with graded expression of α vβ 3 integrin, we found that NGR-bearing Ad bound weakly to α vβ 3 integrin. Treatment of RD cells with TGF-1 up-regulated APN and increased transduction efficacy with Ad bearing NGR within cyclic sequence. Increased transduction is less pronounced for Ad bearing NGR within linear sequence and is likely to be predominantly consequence of moderately increased α vβ 3 integrin expression. Both, APN and α vβ 3 integrin are up regulated in endothelial cells in angiogenesis, therefore NGR-bearing adenoviruses could be suitable vectors for tumor gene therapy.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
