Pregled bibliografske jedinice broj: 25495
In Vitro Assessment of Asbestos Fibers Genotoxicity
In Vitro Assessment of Asbestos Fibers Genotoxicity // Zentralblatt für Hygiene und Umweltmedizin, 199 (1997), 6; 558-567 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 25495 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
In Vitro Assessment of Asbestos Fibers Genotoxicity
Autori
Trošić, Ivančica ; Brumen, Vlatka ; Horvat, Đurđa
Izvornik
Zentralblatt für Hygiene und Umweltmedizin (0934-8859) 199
(1997), 6;
558-567
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
V 79 cell line; chrysotile asbestos; chromosomal aberration; sister chromatide exchange
Sažetak
This study was carried out in order to assess the genotoxic effect of in vitro exposure to commercial chrysotile asbestos. V 79 cell line, known as well-established cellular model, was used for detection of asbestos genotoxic potency. Conventional structural chromosomal aberration analysis and sister chromatide exchange (SCE) method were both used for asbestos genotoxicity assessment. Within the experimental protocol applied, V 79 cells were treated with asbestos in concentrations of 100 and 200um/ml F-10 (HAM) media during 90 days, respectively. Analysis of changes in chromosome structure as well as of cell ploidity was performed each tenth day of experimental course, consecutively. Two hundred well spread metaphases were taken into account for chromosomal aberration analysis. Frequency of sister chromatide exchange was observed in 50 cells per sample. The results of cytogenetic tests revealed structural chromosomal damages, SCE-elavation and changes in cell ploidity. Cytogenetic effect of asbestos obviously depended on dose applied and on the period of incubation. The results of this study suggest that significant cytogenetic changes occurring after asbestos treatment might directly or indirectly be a part of the biological events responsible for eliciting asbestos-induced carcinogenesis.
Izvorni jezik
Engleski
Znanstvena područja
Javno zdravstvo i zdravstvena zaštita
POVEZANOST RADA
Projekti:
00220205
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
Uključenost u ostale bibliografske baze podataka::
- Index Medicus
- Excerpta Medica
- Environmental Mutagen Information Center