Naslov
Encapsulation of new biologically active peptides into liposomes and their immunoadjuvant effect

Autori
Frkanec, Ruža ; Tomašić, Jelka ; Vranešić, Branka ; Halassy, Beata ; Krstanović, Marina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of Peptide Science, Programme of 27th European Peptide Symposium, Sorrento, 31.08. - 06.09.2002 / - , 2002

Skup
27th European Peptide Symposium

Mjesto i datum
Sorrento, Italija, 31.08.2002. - 06.09.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Liposomes; peptidoglycan monomer; adamantyltripeptides; adjuvants

Sažetak
The peptidoglycan monomer (PGM) and synthetic adamantyltripeptides (D- and L-(adamant-2-yl)-Gly-L-Ala-D-isoGln) comprising a part of peptidoglycan structure, were incorporated in large multilamellar negatively charged vesicles using egg lecithin and synthetic dipalmitoyl phosphatidylcholine (DPPC). The entrapment of tested compounds and the stability of the respective preparations were followed in two ways: by the use of 14C-labelled peptidoglycans or by HPLC. The immunoadjuvant activity was tested in vivo, in mice, using ovalbumin (OVA) as an antigen. The production of OVA-specific antibodies was monitored by ELISA. In comparison to the nonentrapped tested compounds the encapsulated preparations showed the better anti-OVA antibody production. Hence, incorporation of immunoadjuvants into liposomes improved their adjuvant activity. In conclusion, liposomes containing immunoadjuvants were prepared, quantity of incorporated material determined and the improved adjuvant effect of immunoadjuvants was demonstrated

Izvorni jezik
Engleski

POVEZANOST RADA