Pregled bibliografske jedinice broj: 252961
Partial purification and characterization of N-Acetylmuramyl-L-alanine amidase from human and mouse serum.
Partial purification and characterization of N-Acetylmuramyl-L-alanine amidase from human and mouse serum. // Biochimica at Biophysica Acta, 701 (1982), 63-71 (međunarodna recenzija, članak, znanstveni)
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Naslov
Partial purification and characterization of N-Acetylmuramyl-L-alanine amidase from human and mouse serum.
Autori
Valinger, Zdenka ; Ladešić, Branko ; Tomašić, Jelka
Izvornik
Biochimica at Biophysica Acta (0167-4838) 701
(1982);
63-71
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Peptidoglycan monomer; N-Acetylmuramyl-L-alanine amidase. mammalian sera
(Peptidoglycan monomer; N-Acetylmuramyl-L-alanine amidase; mammalian sera)
Sažetak
The enzyme N-acetylmuramyl-L-alanine amidase has been detcted in human, mouse, rabbit, bovine and sheep sera. A method for detection of amidase activity using 14-C-peptidoglycan monomer as the substrate has been developed. Partial purification of human and mouse amidase was achieved by gel chromatography. Both amidase preparations exhibited maximal activity at pH 9.0 in Tris-HCl buffer and required Mg2+ for maximal activity. Following digestion of peptidoglycan monomer, the disaccharide GlcNAc-MurNAc and the corresponding pentapeptide L-Ala-D-isoGln-meso-DAP-D-Ala-D-Ala were formed and subsequently isolated and chemically characterized.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti
POVEZANOST RADA
Ustanove:
Institut "Ruđer Bošković", Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
