Pregled bibliografske jedinice broj: 252821
The determination of DNA entrapment into liposomes using short monolithic columns
The determination of DNA entrapment into liposomes using short monolithic columns // Monolith Summer School (MSS 2006) Applications in Biochromatography, Bioconversion and Solid Phase Synthesis : Abstracts / Štrancar, Aleš ; Glover, Darryl (ur.).
Ljubljana: BIA Separation, 2006. str. 26-26 (pozvano predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 252821 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The determination of DNA entrapment into liposomes using short monolithic columns
Autori
Brgles, Marija ; Halassy, Beata ; Tomašić, Jelka ; Forčić, Dubravko ; Šantak, Maja ; Barut, Miloš ; Štrancar, Aleš.
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Monolith Summer School (MSS 2006) Applications in Biochromatography, Bioconversion and Solid Phase Synthesis : Abstracts
/ Štrancar, Aleš ; Glover, Darryl - Ljubljana : BIA Separation, 2006, 26-26
Skup
Monolith Summer Scool
Mjesto i datum
Portorož, Slovenija, 28.05.2006. - 31.05.2006
Vrsta sudjelovanja
Pozvano predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
entrapment efficiency; liposomes; CIM disk
Sažetak
The entrapment of DNA into liposomes enhances the potency of DNA vaccines1. In the process of DNA-containing liposome preparation and characterisation, it is necessary to determine the quantity of DNA entrapped within or complexed to liposome vesicles. The most widely used method is the one that uses the radioactively labelled DNA2. In this study we describe the development of the indirect non-radioactive method using short monolithic columns for the measurement of DNA entrapment that does not require any kind of DNA labelling. Non-entrapped DNA from the supernatant of centrifuged liposomes was chromatographed on CIM DEAE disk and detected by absorbance at 260 nm. The unknown quantity of DNA in samples was calculated from the integrated peak areas using standard curve. Finally, the method was used to determine the efficiency of pCMV (HBs) entrapment within cationic liposomes at various charge ratios. (1) G. Gregoriadis, R. Saffie, B. Souza, FEBS Letters, 402, 107-110 (1997) (2) P.A Monnard, T. Oberholzer, P.L. Luisi, Biochimica et Biophysica Acta, 1329, 39-50 (1997)
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
0021002
Ustanove:
Imunološki zavod d.d.
Profili:
Jelka Tomašić
(autor)
Maja Šantak
(autor)
Beata Halassy
(autor)
Marija Brgles
(autor)
Dubravko Forčić
(autor)