Naslov
Biološki učinci tri novopripravljena spoja na stanične kulture dobivene iz pacijenata oboljelih od Dupuytrenove kontrakture
(Biological Effects of Three Newly Synthesized Compounds on the Cell Cultures from the Patients Affected by Dupuytren's Contracture)

Autori
Bratulić, Siniša ; Jurišić, Davor ; Bujak, M. ; Karminski-Zamola, Grace ; Žinić , Biserka ; Pavelić, Krešimir ; Kraljević Sandra

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
EMBO/HHMI Central European Scientists Meeting

Mjesto i datum
Cavtat, Hrvatska, 15.06.2006. - 17.06.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Dupuytrenova kontraktura; terapija; primarna stanična kultura
(Dupuytren's contracture; therapy; primary cell culture)

Sažetak
Dupuytren's contracture (DC) is a palmar fibromatosis with partially elucidated aetiology. It is characterized by nodular fibroblastic proliferation and accumulation of myofibroblasts giving rise to the permanent contracture of the palm. So far, the only successful treatment was surgical excision or incision of diseased fascia, often accompanied by high recurrence rates. Experimental chemotherapy, such as 5-fluorouracil (5-FU) proved to be useful in acting locally in the palm by inhibiting myofibroblasts proliferation. 5-FU was shown to inhibit both, proliferation and myofibroblast differentiation in DC's cell cultures. We have therefore established primary cell cultures as a model for studying the effects of three newly synthesized chemical compounds on proliferation and differentiation of Dupuytren's fibroblasts/myofibrobalsts. Cells were characterized by proliferation assays, immunocytochemistry and the cell cycle analysis. We have tested cytotoxic and antiproliferatve effects of an N-sulfonylpyrimidine derivative, an amidino substituted benzimidazo(1, 2-a)quinoline derivative and an amido-substituted dithieno-kinolone derivative by MTT-test.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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