Pregled bibliografske jedinice broj: 250842
Comparative susceptibility of a peptidoglycan monomer from Brevibacterium divaricatum and its anhydromuramyl analogue to hydrolysis with N-acetylmuramyl-L-alanine amidase
Comparative susceptibility of a peptidoglycan monomer from Brevibacterium divaricatum and its anhydromuramyl analogue to hydrolysis with N-acetylmuramyl-L-alanine amidase // Journal of chromatography. A, 440 (1988), 405-414 doi:10.1016/S0021-9673(00)94544-8 (međunarodna recenzija, članak, znanstveni)
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Naslov
Comparative susceptibility of a peptidoglycan monomer from Brevibacterium divaricatum and its anhydromuramyl analogue to hydrolysis with N-acetylmuramyl-L-alanine amidase
Autori
Tomašić, Jelka ; Sesartić, Ljuba ; Martin, Stephen A. ; Valinger, Zdenka ; Ladešić, Branko
Izvornik
Journal of chromatography. A (0021-9673) 440
(1988);
405-414
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
peptidoglycan monomer ; anhydromuramyl analogue ; N-acetylmuramyl-L-alanine amidase ; FAB-MS ; HPLC
Sažetak
Peptidoglycan monomer GlcNAc-MurNAc-L-Ala-D-isoGln-mesoDpm-D-Ala-D-Ala from Brevibacterium divaricatum is composed of the disaccharide pepntapeptide containing muramic acid with a reducing end (ca. 90-95%) and of the anhydromuramyl analogue (anhydromuramyl-PGM ; ca. 5-10%) according to the analysis by HPLC and FAB-MS. The two peptidoglycan analogues can not be separated by simple physico-chemical procedures. The enzyme N-acetylmuramyl-L-alanine amidase cleaves the bond between N-acetylmuramic acid and L-alanine in the PGM molecule. It is shown that anhydromuramyl-PGM is also the substrate for this amidase. In a preparation containing both analogues the amidase hydrolyses preferantially PGM rather than anhydromuramyl-PGM.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Temeljne medicinske znanosti, Biotehnologija
POVEZANOST RADA
Ustanove:
Imunološki zavod d.d.,
Institut "Ruđer Bošković", Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
