Naslov
Determination of foetal calf serum in vaccine preparations by immunoradiometric assay and enzyme-linked immunosorbent assay

Autori
Treščec, Anđa ; Valinger, Zdenka ; Smerdel, Stanislav ; Tomašić, Jelka

Izvornik
Biologicals (1045-1056) 21 (1993); 163-168

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
foetal calf serum (FCS); control of vaccines; immunoradiometric assay (IRMA); enzymeimmunoassay (ELISA); 125-I-labelled anti-FCS
(foetal calf serum (FCS); control of vaccines; immunoradio metric assay (IRMA); enzymeimmunoassay (ELISA); 125-I-labelled anti-FCS)

Sažetak
A simple and sensitive immunoradiometric assay (IRMA) for detection of foetal calf serum (FCS) proteins in vaccine preparations has been developed. Polyclonal 125-I-labelled anti-FCS was used for detection. The developed assay allows quantification of FCS (down to 10ng/ml) in vaccine preparations (measles vaccine). An enzymeimmunoassay was also developed based on the same assay two-site sandwich principle where anti-FCS was directly labelled with horse-radish peroxidase. ELISA was comparable in sensitivity and accuracy with IRMA

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti, Biotehnologija

POVEZANOST RADA