Naslov
Dielectric spectroscopy of genomic DNA solutions

Autori
Vuletić, Tomislav ; Dolanski Babić, Sanja ; Tomić, Silvia ; Krča, Sanja ; Ivanković, Dušica ; Griparić, Lorena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European Biophysical Journal with Biohysics Letters / Watts, Anthony - Heidelberg : Springer, 2005

Skup
15th IUPAB and 5th EBSA Intrenational Biophysics Congress

Mjesto i datum
Montpellier, Francuska, 27.07.2005. - 01.09.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
DNA dielectric relaxation; dielectric spectroscopy of biomaterials

Sažetak
Transport of electrical signals in complex biosystems, and in particular in the key molecule of DNA is of high interest. The anisotropic structure of DNA implies that the signaling process should be extremely sensitive to the local environment of the double helix. Revealing the dynamical properties of DNA as a function of its aqueous environment is certainly fundamental for macromolecular mechanisms in the cell. Low frequency dielectric spectroscopy was employed, in the frequency range of 100 Hz to 100 MHz. This is a non-destructive tool, which can be used for detecting and quantifying the polarization response of DNA in aqueous environment. A wide concentration range of salmon sperm and calf-thymus Na-DNA solutions in pure water, and in NaCl electrolyte of varying ionic strength was investigated. We discuss the possible assignments of observed relaxation modes taking into account the value and the concentration dependence of the dielectric relaxation parameters obtained from fits to the generalized Debye function. Persistence length is identified along with other characteristic length scales. We also discuss the influence of DNA on its environment, that is, on the effective diffusion constants of ions in solution.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Biologija

POVEZANOST RADA