Naslov
Age related changes in bone mineral content, bone mineral density and ash density of the vertebral bone in relation to the bone volume in women

Autori
Zoričić, Sanja ; Cvijanović, Olga ; Kristofić, Ines ; Bobinac, Dragica ; Crnčević-Orlić, Željka ; Marić, Ivana ;

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 29th European Symposium on Calcified Tissues ; u: Calcified Tissue International 70 (2002) (4) 242-292 ; P-68 / - , 2002, 277-277

Skup
European Symposium on Calcified Tissues (29 ; 2002)

Mjesto i datum
Zagreb, Hrvatska, 25.05.2002. - 29.05.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
vertebral bone; bone mineral density; ash density; bone volume

Sažetak
The aim of the present study was to determine the age-related changes in vertebral bone structure in women assessed by three different methods: dual energy X ray absorptiometry (DEXA), as density analysis and bone histomorphometry. Furthemore, numerical ratios and correlations between measured bone parameters were calculated in order to establish true relations between them. The lumbar vertebral bodies without posterior element (L3) from normal female cases were collected during autopsy. The samples were divided into three age related groups of ages: 50-60, 60-70, and 70-80. The vertebral bodies without posterior elements, were carefully removed and cleaned from the surrounding tissue, washed in saline solution and frozen on -20 deg C until performing the measurement. Each specimen was placed in container filled with demineralized water and scanned with Hologic QDR 4500 C sacanner in a vertical plane under constant conditions. The density calculations (BMC and BMD)were made with the scanning software in the default mode. Thereafter, 3 vertical bone cylinders were obtained from each specimen using bone trephine. Two cylinders were embedded undecalcified in MMA, cut on 5 microm thick slices, histomorphometrically stained with toluidin blue. Image analysis was performed using semiautomatic image analyzer (Sform, VAMS, Zagreb) and BV/TV was calculated. The third cylindrical bone specimen was ashed (580 deg C for 24 h) and weighed. Apparent ash density (AD)was calculated by dividing ash weight by the volume for the specimen. Calculating numerical relations between BMS, BMD, ash density and BV/TV showed constant ratios. These ratios did not significantly differ between different age groups. The results revealed that histomorphometrically calculated bone volume (BV/TV) was in accordance with the mineral content and density of the vertebral bone.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA