Naslov
Interactions of quercetin and its lanthane complex with double stranded DNA / RNA and single stranded RNA. Spectrophotometric sensing of poly G.

Autori
Marinić, Mirna ; Piantanida, Ivo ; Rusak, Gordana ; Žinić, Mladen

Izvornik
Journal of Inorganic Biochemistry (0162-0134) 100 (2006), 2; 288-298

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
quercetin; DNA; RNA; lanthane

Sažetak
Spectrophotometric titrations revealed that stability of the quercetin / ds- DNA or RNA non-covalent complexes is significantly higher compared to the quercetin / ss- RNA complexes. This observation can easily be correlated with the significantly larger aromatic surface of base pairs compared to single nucleobases, and it is in good agreement with other experimental data pointing toward intercalative binding mode of quercetin. Fluorescence increase of quercetin induced by ds-RNA is significantly stronger than observed for ds-DNA, offering usage of quercetin as the ds-RNA selective fluorescent probe. Also, addition of poly G yielded more than order of magnitude stronger changes in UV/vis and fluorescence spectrum of quercetin compared to the changes upon addition of poly A and poly U revealing possible usage of quercetin as a powerful spectroscopic probe for poly G sequences. Stability and stoichiometry of Lanthane3+/quercetin complexes in physiologically relevant aqueous media was determined. The interactions of (LaQ)3+ with double stranded DNA and RNA were significantly different compared to the free quercetin, revealing increase of complex stability and thus significant impact of La3+ in binding of (LaQ)3+ to polynucleotides. Similar results were observed for interactions of (LaQ)3+ with single stranded RNA.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

Napomena
Online http://www.sciencedirect.com/science/journal/01620134

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