Naslov
Expression of chemokine receptor CX3CR1 in infants with respiratory syncytial virus bronchiolitis

Autori
Cepika, Alma-Martina ; Gagro, Alenka ; Baće, Ana ; Tješić-Drinković, Dorian ; Kelečić, Jadranka ; Voskresensky-Baričić, Tamara ; Matić, Mladen ; Draženović, Vladimir ; Marinić, Igor ; Mlinarić-Galinović, Gordana ; Tješić-Drinković, Duška ; Vrtar, Zvonimir ; Rabatić, Sabina

Izvornik
Pediatric Allergy and Immunology (0905-6157) 19 (2008), 2; 148-156

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
infants; RSV; bronchiolitis; CX3CR1; perforin; IFN-gamma

Sažetak
Respiratory syncytial virus (RSV) glycoprotein G mimics fractalkine, a CX3C chemokine, which mediates chemotaxis of leukocytes expressing its receptor, CX3CR1. The aim of this study was to examine the relationship between RSV infection and expression of perforin and IFN-gamma in CX3CR1-expressing peripheral blood CD8+ T cells. Samples were collected from infants with RSV bronchiolitis, both in the acute and convalescence phase (n = 12), and from their age- and sex-matched healthy controls (n = 15). Perforin expression and IFN-gamma secretion in CX3CR1+ CD8+ T cells were assessed by four-color flow cytometry. The NF-kappaB p50 and p65 subunit levels were also determined as markers of RSV-induced inflammation. Study results showed perforin and CX3CR1 expression to be significantly lower in the convalescent phase of infected infants than in healthy controls. There was no significant difference in IFN-gamma secretion and NF-kappaB binding activity between two time-points in RSV-infected infants, or when compared with healthy controls. Infants with prolonged wheezing had lower acute-phase CX3CR1 levels in peripheral blood. These data indicate existence of an event persisting after acute RSV infection that is able to modulate effector functions of cytotoxic T cells, and also link disease severity with CX3CR1 expression.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA