Naslov
The use of vascularized bone created by epigastric flap prefabrication with Bone morphogenetic protein 7 for reconstruction of long bone defects in the rat model

Autori
Žic, Rado ; Martinović, Snježana ; Kušec, Vesna ; Vlajčić, Zlatko ; Stanec, Sanda ; Stanec, Zdenko ; Vukičević, Slobodan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10th Congress of ESPRAS, Abstract book / Frey, Manfred - Beč, 2005

Skup
10th Congress of ESPRAS

Mjesto i datum
Beč, Austrija, 30.08.2005. - 03.09.2005

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
epigastric flap prefabrication; BMP-7; large bone defect

Sažetak
Introduction Repair of large bone defects can still be a problem. If we could prefabricate the needed bone size and shape in any standard flap we would greatly expand our reconstructive options and reduce the donor site morbidity thus improving the overall functional and aesthetic result. In this study we investigated the possibility of prefabrication of bone in the epigastric flap of the rat using Bone morphogenetic protein 7 (BMP7) and the usefulness of the prefabricated vascularized bone in reconstructing long bone defects of the rat femur. Material and Methods A hundred and eight male Wistar rats used in this study were divided into six groups. Collagen sponge or dematerialized bone was treated with a low (30µ g) or high (120 µ g) dose of BMP7 or with diluent only. The carrier was then inserted under the epigastric flap in the rat groin. After six weeks, when the new bone was formed, a gap of 0.8 cm was formed in the ipsilateral femur of each rat. This defect was reconstructed with the bone in the prefabricated epigastric flap. Eight weeks after the defect was reconstructed the animals were sacrificed. The healing in different groups was assessed by X-ray, examination and histology. Results BMP-7 stimulated bone formation in both the collagen sponge and demineralized bone implants irregardless of the dose. Histology of the femur specimens showed healing of bone defect reconstructed with the vascularized prefabricated bone in the high dose BMP-7 groups. Non-union was found in most femurs reconstructed with flaps that were prefabricated with low BMP-7 dose implants and all femurs reconstructed with flaps prefabricated with diluent treated implants. Conclusion The results of this study suggest bone can be successfully formed in a soft tissue flap using BMP7 and used to reconstruct long bone defects which would otherwise result in non union.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Veterinarska medicina

POVEZANOST RADA