Naslov
Regulatory T cells in children allergic to house dust mite undergoing specific immunotherapy

Autori
Ajduk, Jakov ; Aberle, Neda ; Rabatić, Sabina ; Gagro, Alenka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Abstract book, OGAI (Austrian Society for Allergology and Immunology) Annual Meeting 2003 / - Salzburg, 2003

Skup
OGAI Annual Meeting 2003

Mjesto i datum
Salzburg, Austrija, 04.12.2003. - 06.12.2003

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
regulatory T cells;
(regulatory T cells; immunotherapy; allergen)

Sažetak
Regulatory T cells (Treg) are defined by their surface phenotype (CD4+CD25+CD45RO+CD69-CTLA-4+) or by their ability to secrete immunoregulatory cytokines, such as interleukin (IL)-10 and transforming growth factor (TGF)- β . The aim of this study was to detect Treg in peripheral blood from children allergic to Dermatophagoides pteronyssinus undergoing specific immunotherapy (n=15). Treg were identified using four-color flow cytometry prior to, after three months, and after one year of immunotherapy. In addition, intracellular detection of IL-10 and TGF- β in T cells was performed. The level of CTLA-4 expressing Tregs increased transitory after 3 months of immunotherapy. While no changes in IL-10 secreting T-cells were found, specific immunotherapy increased percentage of TGF-β secreting T cells. These results indicate that analysis of CTLA-4 and TGF- β by flow cytometry could be useful markers to monitor the effect of specific immunotherapy in allergic individuals.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA