Naslov
Fine needle aspiration cytology of hepatic and pancreastic tumours

Autori
Kardum-Selin, Ika ; Šušterčić, Dunja ; Fabijanić, I ; Jelić-Puškarić, B ; Kušec, Rajko ; Kardum-Paro, MM ; Planinc-Peraica, Ana ; Ostojić-Kolonić, Slobodana ; Odak, D ; Anić, P ; Škegro, Dinko ; Čolić-Cvrlje, Vesna ; Papa, Branko ; Jakšić, Branimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka 3. Hrvatskog kongresa patologije i sudske medicine.3. Hrvatski kongres kliničke citologije. 1. Hrvatski simpozij citotehnologije / Jonjić, Nives ; Kardum-Skelin, Ika - Karlovac, 2005

Skup
3. Hrvatskog kongresa patologije i sudske medicine.3. Hrvatski kongres kliničke citologije. 1. Hrvatski simpozij citotehnologije

Mjesto i datum
Opatija, Hrvatska, 08.05.2005. - 11.05.2005

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cytologic fine needle aspirates

Sažetak
Imaging methods (ultrasound-US, computerised tomography-CT and magnetic resonance-MR) have ensured the visualisation of intraabdominal tumours (of liver, pancreas, kidneys, adrenal glands and retroperitoneal nodes) which are very rarely approachable on palpation, and consequently in guided aspiration. Aim of the study: 1. To analyse the adequacy of cytologic fine needle aspirates from intraabdominal organs and retroperitoneal nodes, 2. To estimate the occurrence rate of malignant lesions, 3. The possibility for typisation of epithelial and nonepithelial tumours by cytomorphology and additional technologies from a cytologic specimen. Patients and Methods: 1528 intraabdominal aspirates were examined: liver (696), pancreas (289). Fine needle aspiration was performed with the CHIBA needle. The preparations were analysed in standard stained smears by the May-Grunwald-Giemsa method, by determination of cellular markers by immunocytochemical analysis on smears and flow cytometry, cytochemical, cytogenetic and/or molecular analyses (PCR). Results: In 1480 patients fine-needle aspiration was performed with the US control, in 12 patients with CT, and in only 36 patients without image control. Insufficient material was obtained in 99 patients (6, 5%). Malignant cells were found in 648 cases in aspirates of both liver and pancreas. Of a total of 528 liver tumours, based on cytomorphology, completed with immunocytochemical markers, the epithelial, mesenchymal or embryonal cellular origin could be determined (92% epithelial, 6, 6% nonepitehelial origin and 1, 4% with suspicious features without precise differentiation of origin and/or type of tumours). In the tumors of epithelial group primary epithelial liver tumours numbered 21, 4% (101 hepatocellular and 3 cholangiocellular carcinoma), plus 306 metastatic ones, while in the epithelial tumors primary site was recognised in 15, 5% cases. In poorly differentiated tumors, the tumor aggressiveness was confirmed by high aneuploidy (DNA image cytometry) and by high proliferation status (AgNOR determination). In nonepithelial tumours the diagnosis of gastrointestinal stromal tumor (GIST) was made in 2, embryonal sarcoma in 1, angiosarcoma in 1, schwannoma in 1, leiomyosarcoma in 1, fibrosarcoma in 3, melanoma in 9 and 15 malignant lymphomas, while others could be diagnosed as sarcomas without precise differentiation. Out of 121 diagnosed malignant tumours of pancreas we found 112 carcinomas, 7 suspicious specimens and 1 mesenchymal tumour. Conclusion: Cytodiagnostic fine-needle aspiration of the liver and pancreas, completed with immunocytochemical markers on smears and flow cytometry, by cytogenetical and molecular analyses from the specimens obtained by cytologic aspiration, has been proved to be a reliable method in diagnostics and subtypisation of epithelial (primary as well as metastatic) and nonepithelial tumors. However, very often, some tumor markers reveal tissue co-expression and, as a rule, are not specific for only one organ. Despite this, their right combination improves the search for the primary site of the metastatic process or, potentially determine the additional diagnostic procedures (CT, US, MR).

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA