ࡱ> %'$7  bjbjUU .7|7|llllllll  Z\\\\\\$ X!l4ll444llZ4Z44NllN RZXT8XNN 0N 4 N4llllEXPOSURE TO HYPEBARIC PRESSURE ALTERS GANGLIOSIDE EXPRESSION IN RAT LIVER FOLLOWING PARTIAL HEPATECTOMY A. Markoti, V. ikea uli, T. Ti inovi Kurir, S. Radi, T. Zemunik, M. Mesari Department of Biochemistry, Medical School, Split University, `oltanska 2, HR 21000 Split, Croatia Introduction: Remnant liver after partial hepatectomy (PH) demands an increased amount of oxygen to restore hepatic energy. Modulation of the ganglioside content of hepatocyte plasma membrane could provide a mean for upregulating cellular responsiveness to a variety of factors. Materials and Methods: Rat liver gangliosides were analysed by high perfomance thin layer chromatography (HPTLC) followed with immunostaining 54 h after PH. Non-operated and three different groups of operated rats were studied according to the hausing conditions before or after operation: recovering under normal ambient conditions after operation (1), treating with hyperbaric pressure before operation and recovering under normal ambient conditions (2 or preHB), and recovering under hyperbaric pressure after operation (3 or postHB). Results: Liver of preHB group showed the highest expression of ganglioside GD1a, a marker of a peculiar transition phase of liver regeneration. Same animals showed enhanced expression of the several gangliosides: GM2, GM1a, GD1b, GT1b, GalNAcGM1b, nLc4, nLc6 and nLc8. Conclusion: Since galactosyl- and N-acetylgalactosaminyltransferase catalyse final step of synthesys of GM2, GM1a, GD1b, GalNAcGM1b, nLc4, nLc6, and nLc8, our results point at possible role of galactosyl- and N-acetylgalactosaminyltransferase in regulation of the liver growth after PH. !"2:;z\ X mH sH  mHnHu H*mHsHmHsH >*mHsH5CJOJQJ\^J5OJQJ\^Jz|!";V e $a$$da$ ,1h. A!"#$%  i8@8 NormalCJ_HaJmH sH tH LL Heading 7$d@&5OJQJ\^JmHsH<A@< Default Paragraph FontFB@F Body Textdh5OJQJ\^JmHsH.U@. Hyperlink >*B*phjk!";Ve00000000000000   ik $;U33Anita MarkotiHC:\Documents and Settings\x\My Documents\hdf Markotic et al abstract.docAnita MarkotipC:\Documents and Settings\x\Application Data\Microsoft\Word\AutoRecovery save of hdf Markotic et al abstract.asdx/C:\My Documents\hdf Markotic et al abstract.doc@$d@@ @UnknownG:Times New Roman5Symbol3& :Arial"1hs&.c !202QAbstractAnita Markotix Oh+'0t  0 < HT\dl Abstract bstAnita Markotinitnit Normal.dottxrm15mMicrosoft Word 9.0@40@.:R@GXTc ՜.+,0 hp|  a   Abstract Title  !"#&Root Entry F:[ZXT(1Table WordDocument.SummaryInformation(DocumentSummaryInformation8CompObjjObjectPool:[ZXT:[ZXT  FMicrosoft Word Document MSWordDocWord.Document.89q