Naslov
Effects of hydrocortisone and dexamethasone on galectin-3

Autori
Dabelić, Sanja ; Dumić, Jerka ; Flögel, Mirna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Third Croatian Congress on Pharmacy / Zorc, Branka - Zagreb, 2005

Skup
Third Croatian Congress on Pharmacy

Mjesto i datum
Cavtat, Hrvatska, 27.04.2005. - 30.04.2005

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
hydrocortisone; dexamethasone; galectin-3

Sažetak
Galectin-3, a 29-32 kDa  -galactoside binding lectin, is implicated in many biological processes, (e.g. growth regulation, pre-mRNA splicing, cell differentiation, neoplastic transformation and tumor metastasis). It plays a key role in immune responses by modulating T-cell apoptosis, proliferation, cell adhesion, chemotaxis and synthesis of inflammatory mediators such as cytokines, nitric oxide and prostaglandines. Galectin-3 expression is influenced by various stimuli but the precise regulatory mechanisms are not yet elucidated. In our previous studies we showed the involvement of the NF-kappaB and AP-1 transcription factors in regulation of galectin-3 expression. It was also shown that galectin-3 expression in macrophages is regulated by Ras/MAP kinase signalling pathway. The mentioned transcription factors and signalling pathway are strongly influenced by many anti-inflammatory drugs. Therefore, the aim of our study was to determine the effects of steroid immunomodulatory drugs (hydrocortisone and dexamethasone) on the expression of galectin-3 both on the mRNA and protein level. The human monocytic cell line THP-1 was exposed to various concentrations of hydrocortisone (0.1, 1, 5 and 10 μ M) and dexamethasone (0.1, 1, 10 and 100 nM) during 1, 3, 5, 24, 48 or 72 hrs. The target gene mRNA level is evaluated using relative RT-PCR method and GeneScan analysis software. The results showed that all concentrations of applied drugs halved the galectin-3 mRNA level already after 1 h of exposure and that the level of mRNA continued to decrease during time and was only 15, 4-3, 8% of the initial value after 72 h. Surprisingly, the protein expression measured by chemiluminescent-western blot analysis didn’ t follow the same pattern – the decrease was observed only after 48 and 72 h of exposure, when the galectin-3 level fall on 71% - 38, 7% of the initial value, respectively.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA