Naslov
Tumor-associated Proteolytic Factors uPA and PAI-1 in Thyroid Carcinoma

Autori
Horvatić Herceg, Gordana ; Herceg, Davorin ; Bence-Žigman, Zdenka ; Tomić-Brzac, Hrvojka ; Kralik, Marko ; Kulić, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the Annual Congress of the EANM, Istanbul, Turkey 2005, European journal of nuclear medicine and molecular imaging, 32, 1 / - , 2005, S238-s238

Skup
Annual Congress of the EANM

Mjesto i datum
Istanbul, Turska, 15.10.2005. - 19.10.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
plasminogen activator system ; thyroid cancer ; ELISA

Sažetak
There is abundant experimental evidence that the plasminogen activator (PA) system with the key components, the serine protease uPA (urokinase-type plasminogen activator), its inhibitor PAI-1 (plasminogen activator inhibitor type-1), and its cell surface receptor (uPA-R, CD 87), plays a fundamental role in tumor invasion and metastasis. A large body of clinical data indicates that high levels of uPA and PAI-1 can be used to predict poor patient prognosis for multiple types of solid tumors. In thyroid cancer very intensely and diffusely uPA and PAI-1 immunohistochemical expression have been described, but no relationship between the expression of these proteins and clinicopathological parameters could be determined. Therefore, we evaluated whether the expression and activation of uPA and PAI-1 might be of clinical value as a tumor/biologically defined risk factor in patients with thyroid carcinoma. The levels of uPA and of its inhibitor (PAI-1) were measured by use of ELISA in the cytosol of tissue homogenates obtained from thyroid cancer and tumor-free thyroid in 23 pts (median 56 y, range 3-76, 18 female, 5 male). All pts were staged according to tumor size, nodal involvement, distant metastasis and other relevant predictor of prognosis. Significantly higher mean value levels of uPA and PAI-1 were found in thyroid carcinoma (uPA 1, 110 ng/mg, PAI-1 15, 036 ng/mg) compared to tumor-free thyroid (uPA 0, 004 ng/mg, PAI-1 2, 349 ng/mg). Both uPA and PAI-1 were significantly different in various histologic grades (p< 0, 024, p< 0, 017, respectively). The uPA and PAI-1 results show significant differences among thyroid histological type and multicentricity. However no significant association between uPA and PAI-1 levels and extrathyroid invasion was found. There was a correlation between the levels of uPA and PAI-1 expression in cancerous tissue (p<0, 001, correlation coefficient =0, 72). We found significant correlation between uPA (urokinase-type plasminogen activator), its inhibitor PAI-1 (plasminogen activator inhibitor type-1) and the standard prognostic parameters in thyroid cancer. Further investigations have to clarify whether uPA and PAI-1 could be an independent prognostic factor.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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