Pregled bibliografske jedinice broj: 211142
Follicular dynamics and in vitro embryo production after repeated superovulation with FSH-P in Simmental heifers.
Follicular dynamics and in vitro embryo production after repeated superovulation with FSH-P in Simmental heifers. // Proceedings of the 6th Middle-European Buiatrics Congress, 1-4 June 2005, Cracow, Poland / Edward Malinowski, Tadeusz Wijaszka (ur.).
Krakov: National Veterinary Researdh Institute in Pulawy, 2005. str. 381-387 (predavanje, međunarodna recenzija, cjeloviti rad (in extenso), znanstveni)
CROSBI ID: 211142 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Follicular dynamics and in vitro embryo production after repeated superovulation with FSH-P in Simmental heifers.
Autori
Getz Iva, M. Samardžija, M. Karadjole, Z. Makek, Lj. Bedrica, T. Karadjole, D. Gračner
Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni
Izvornik
Proceedings of the 6th Middle-European Buiatrics Congress, 1-4 June 2005, Cracow, Poland
/ Edward Malinowski, Tadeusz Wijaszka - Krakov : National Veterinary Researdh Institute in Pulawy, 2005, 381-387
Skup
6th Middle European Buiatrics Congress
Mjesto i datum
Kraków, Poljska, 01.06.2005. - 04.06.2005
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
heifer; superovulation; ovum pick-up; oocytes; in vitro culture; developmental competence.
Sažetak
Ultrasound transvaginal ovum pick-up was performed in 6 Simmental heifers aged between 14 and 18 months. Heifers were synchronized with PGF2 and stimulated with pFSH, twice a day during two days (Folltropin® ; ; ; ; ; ; -V, Vetrepharm Inc., London, Canada, a total dose: 200 mg NIH-FSH-P1). Dominant follicle presence was registered ultrasonically on the beginning of superovulatory treatment and follicular dynamic was followed-up 12, 24 and 48 hours after the first pFSH injection. Follicles were classified according to diameter in 4 categories: 2-5 mm ; 6-9 mm ; 10-14 mm and  15 mm. OPU was performed 48 hours after the last FSH injection and procedure was repeated every 14 days during 2 months (a total of 4 aspirations per heifer). Retrieved oocytes were classified in four quality categories. Grade 1 and grade 2 oocytes were matured, fertilized and cultured in vitro in SOFaaBSA medium till the Day 10. Cultured embryos were evaluated morphologically according to IETS standards. Presence of dominant follicle, registered in 10 heifers, did not affect follicular dynamics after repeated superovulation treatments. The mean number of 4 diameter categories follicles did not show significant differences between repeated superovulation treatments. The mean number of grade 1 and grade 2 categories oocytes was 5.67 0.89. The mean number of 3rd and 4th category oocytes were: 1.37 0.38, 0.25 0.11, respectively. In vitro culture results were: 82.13% cleavage rate on Day 2, 43.14% of morula/blastocysts on Day 7 and 33.08% of hatched blastocysts on Day 10. The results show that the repeated FSH stimulation prior to ovum pick-up does not affect the follicular dynamic and developmental competence of bovine oocytes in Simmental heifers.
Izvorni jezik
Engleski
Znanstvena područja
Veterinarska medicina
