Naslov
Genotypic and phenotypic diversity of lactic acid bacteria isolated from controlled sauerkraut fermentation

Autori
Beganović, Jasna ; Kos, Blaženka ; Gjuračić, Krešimir ; Šušković, Jagoda ; Frece, Jadranka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final Programme and Book of Abstracts of the Second Congress of Croatian Geneticists / Franekić-Čolić, Jasna ; Ugarković, Đurđica - Zagreb, 2005

Skup
Second Congress of Croatian Geneticists with international participation

Mjesto i datum
Supetar, Hrvatska, 24.09.2005. - 27.09.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Lactobacillus plantarum; Leuconostoc mesenteroides; starter cultures; sauerkraut production; PFGE; SDS-PAGE; probiotics

Sažetak
The increasing application of probiotic cultures in food products underscores the need to properly identify and distinguish these beneficial bacteria among originally presented microbial population. Probiotic strain Lactobacillus plantarum L4 and Leuconostoc mesenteroides LMG 7954 were introduced as starter cultures in sauerkraut production for improvement of fermentation process and product quality. Lower biogenic amine content and shorter duration of fermentation were obtained in production of sauerkraut with starter cultures. To check out the influence of starter culture strains, ten randomly chosen lactic acid bacteria isolates from controlled fermentation were genotyped by pulsed-field gel electrophoresis (PFGE), together with six reference strains for comparison. Electrophoretic karyotype of NotI-digested genomic DNA revealed 6 isolates as Lactobacillus plantarum, one was identified as Leuconostoc mesenteroides and one as possible Lactobacillus rhamnosus. The two remained isolates showed specific karyotype that could not be identified after comparison with reference strains. Results obtained by PFGE were confirmed by SDS-PAGE of whole cell proteins. Further phenotypic characterization of all strains examined was evaluated by studying biochemical characteristics (API carbohydrate fermentation patterns and acidifying activities) of technological interest. The results indicated that electrophoretic karyotype determined by PFGE allowed to confirm the identity of starter strains between other isolates and can be employed to study the genetic diversity of lactic acid bacteria from an ecosystem like that found in fermenting vegetables.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA