Naslov
RT-PCR and PCR detection of pestiviruses and porcine circovirus type 1 in swine vaccines as additional methods for vaccine quality control

Autori
Jemeršić, Lorena ; Terzić, Svjetlana ; Lojkić, Mirko ; Čač, Željko ; Roić, Besi ; Cvetnić, Željko ; Keros, Tomislav ; Špičić, Silvio

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of the International Conference New diagnostic Technology, Aplications in animal health and biologics controls / Vannier, J.P. ; Espeseth, D. - Ploufragan : OIE, 2005, 103-103

ISBN
978-3-8055-8116-5

Skup
New diagnostic Technology, Aplications in animal health and biologics controls

Mjesto i datum
Saint-Malo, Francuska, 03.10.2005. - 05.10.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
RT-PCR; PCR; pestiviruses; porcine circovirus type 1; swine vaccines

Sažetak
Pestiviruses, primarily bovine viral diarrhea virus (BVDV), have been identified as the most common contaminants of fetal calf sera (FCS) and cell cultures of different origin used for veterinary vaccine production. Eventhough pigs are not natural hosts of BVDV, inoculation of BVDV contaminated attenuated vaccines may cause clinical disease and/or disturb pestivirus detection and diagnosis. Porcine circovirus type 1 (PCV1) has also been identified as a common contaminant of cell cultures originating from pig tissues which are used for viral growth and vaccine production. Eventhough PCV1 is non pathogenic for pigs, PCV2 with similar antigenic properties is a cause of severe diseases in pigs named post-weaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS). In this study we are presenting the results of cell culture, FCS and vaccine testing by RT-PCR and PCR that was carried out in order to evaluate the contamination of experimental and commercially available pig vaccines.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

Napomena
Developments in Biologicals, Vol. 126 ; ISSN 1424-6074

POVEZANOST RADA