Naslov
Botryotinia fuckeliana isolates carrying different transposones show differential response to fungicides and localization on host plant

Autori
De Miccolis, Angelini Rita ; Miličević, Tihomir ; Natale, Patrizia ; Lepore, Antonio ; De Guido, Maria Antonia ; Pollastro, Stefania ; Cvjetković, Bogdan ; Faretra, Franco

Izvornik
Journal of plant pathology (1125-4653) 4 (2003), 85; 288-289

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Botryotinia fuckeliana; transposons; fungicides; host plants

Sažetak
Gray mould, caused by Botryotinia fuckeliana (de Bary) Whetz (teleomorph of Botrytis cinerea Pers.), causes heavy yield losses on many crops all over the world. The fungus shows a broad phenotypic variability. Several sources of variation are known, such as heterokaryosis, heteroploidy, transposable elements, and dsRNA and virus-like particles. Two transposable elements (Boty and Flipper) have been found in B. fuckeliana. Two coexisting sympatric populations of the fungus, transposa (Boty+ Flipper+) and vacuma (Boty- Flipper-) with different host-specialisation and response to fungicides, were recognized in France. 530 isolates from different plant hosts and geographical origins were investigated through PCR reactions using primers specific for the two transposons. 352 isolates were Boty+ Flipper+, 45 were Boty+ Flipper-, 20 Boty- Flipper+, and 113 Boty- Flipper-. Isolate representative of B. fuckeliana populations present on grapevine and strawberry in Italy and Croatia were sampled during the growing season. Response to fungicides (benomyl, viclozolin, pyrimethanil, fludioxinil, fenhexamid, and dichlofluanide) of isolates was evaluated in colony growing tests. On fruits, Boty+ Flipper+ and Boty+ Flipper- isolates were largely prevalent on Boty- Flipper- isolates, while they were in similar proprtions on other plant organs. The mayority of Boty+ Flipper+ (70 %) and Boty+ Flipper- (55 %) isolates were resistant to one or more fungicides, while the majority of Boty- Flipper+ (70 %) and Boty- Flipper- (85 %) isolates showed the wild-type sensitive phenotype. Further investigations are in progress on the role of the various types of B. fuckeliana isolates in the epidemiology of gray mould and fungicide resistance

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA